Addgene

We narrowed to 6,225 results for: sms

Showing: 1681 - 1700 of 6225 results

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delta NT delta CT p70S6K1 MSCV puro

Plasmid

#8463

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta NT delta CT p70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

p70 s6k1 form

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only
deltaCT S6K1 MSCV puro

Plasmid

#8465

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta ct p70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids

Promoter

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pALPS puro miR30-IRF9

Plasmid

#117159

Purpose

Target site AATTATCACAAAGAGGACAGG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (D14A/G38A)

Plasmid

#128118

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (D14A/G38A)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBP-TL9

Plasmid

#74098

Purpose

TL9 RBS (AAAAAG)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

TL9 RBS

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Sestrin2-E451A

Plasmid

#73674

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-HA-Sestrin2-L261A

Plasmid

#73672

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
YCe1901 HC_Kan_PGKpA_p22

Plasmid

#100683

Purpose

part designed to occupy position 22 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

PGKpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Flu cPitx2 (CT#704)

Plasmid

#13928

Depositor

Cliff Tabin

Article

Logan et al Cell. 1998 Aug 7. 94(3):307-17.

Insert

pitx2 (PITX2 Chicken)

Use

Retroviral; Avian expression

Tags

Flu (3xHA)

Expression

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

Plasmid

#200637

Purpose

Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

Vif

Use

Tags

Expression

Mammalian

Mutation

T27stop

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-2xmNeonC

Plasmid

#178463

Purpose

A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag and C-terminal cysteine in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen dimer

Use

Tags

6xHis

Expression

Bacterial

Mutation

Free cysteine at the C-terminus of the dimer

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCZGY2727

Plasmid

#193330

Purpose

Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrI

Depositor

Yishi Jin

Article

Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

Insert

Hygromycin resistance

Use

Tags

Expression

Worm

Mutation

Promoter

Prps-0

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCZGY2748

Plasmid

#193331

Purpose

Site specific CRISPR/Cas9 editing of C. elegans Chr I

Depositor

Yishi Jin

Article

Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

Insert

sgRNA for ttTi4348

Use

Tags

None

Expression

Worm

Mutation

Promoter

U6

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG CAG CCL20-pre-mGRASP

Plasmid

#173114

Purpose

Expresses CCL20 sender construct in mammalian cells

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCL20-pre-mGRASP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 CMV myc-Arrestin-TEVp

Plasmid

#173115

Purpose

Expresses arrestin receiver construct in mammalian cells

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

myc-Arrestin-TEVp

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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p-preSufI(IAC)

Plasmid

#168516

Purpose

Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

pre-SufI(IAC)

Use

Tags

6xHisC tag and HSV tag

Expression

Bacterial

Mutation

C17I and C295A

Promoter

T7

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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p8130 MSCV GFP Puro C-FlagHA

Plasmid

#184521

Purpose

Expression of GFP

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

GFP

Use

Retroviral

Tags

Flag, HA

Expression

Mutation

Promoter

MSCV LTR

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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NK85

Plasmid

#176611

Purpose

To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.

Depositor

Meghan Morrissey

Article

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

SNAP-FCERG-mGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

C25A

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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NK83

Plasmid

#176610

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

NeonGreen-tSH2 Syk (Syk Mouse, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

n/a

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pIB1884-ximD-PCPT

Plasmid

#169109

Purpose

Production of marmesin in Streptomyces xiamenensis

Depositor

Minjuan Xu

Article

Bu et al ACS Synth Biol. 2020 Sep 18;9(9):2282-22

Insert

XimD and PcPT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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