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pWZL-Stat5a1*6

Plasmid

#130669

Purpose

Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

Depositor

Gerardo Ferbeyre

Article

Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

Insert

Stat5a (Stat5a Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Histidine 298 to Arginine and Serine 710 …

Promoter

LTR

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES-hSphK2-EGFP

Plasmid

#84371

Purpose

Expression of exogenous human SphK2 in mammalian cells

Depositor

Anthony Don, Hugh Rosen

Article

Don et al J Biol Chem. 2007 May 25;282(21):15833-4

Insert

SPHK2 (SPHK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt11-V5

Plasmid

#35941

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt11 (wnt11f2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAtipiTrc

Plasmid

#53275

Purpose

Contains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al J Bacteriol. 2000 Oct;182(20):5841-8.

Insert

ipi1 (IPP2 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Trc

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

Plasmid

#45578

Purpose

Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSSN6

Plasmid

#104340

Purpose

Express SSN6

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

SSN6 (CYC8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt4-V5

Plasmid

#35929

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation C (492) --> T

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP2-Grx2(yeast)

Plasmid

#65005

Purpose

Yeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

GRX2

Use

Tags

roGFP2

Expression

Yeast

Mutation

Promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt2-V5

Plasmid

#35925

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (816) --> A

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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KG#59

Plasmid

#110929

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Article

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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Pinco-mNrp1dCy

Plasmid

#21936

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Expression

Mutation

deleted amino acids 884-923

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
Pinco-mNrp1dSEA

Plasmid

#21935

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Expression

Mutation

deleted amino acids 920-923

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pUCBB-ptet-eGFP

Plasmid

#32554

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Mutation

Promoter

ptet

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pSB1K3_pBen-sfGFP_J23101-BenR

Plasmid

#128125

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP and BenR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBen and J23101

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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TTR C10S S100C

Plasmid

#190010

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S100C

Promoter

t7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiR_Luc-A

Plasmid

#25765

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET5

Plasmid

#107437

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET5 (MET5 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

Promoter

GAL1

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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KG#470

Plasmid

#110930

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Article

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101107

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAct5C-Hh

Plasmid

#37382

Depositor

Norbert Perrimon

Article

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

hedgehog (hh Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

actin5C

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only