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Plasmid#184675PurposeNIR-Fb to GFPDepositorInsertNIR-FbGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-FKBP12DD C-term (w350-2)
Plasmid#17413PurposeGateway entry vector for Degron, C-termDepositorTypeEmpty backboneUseEntry vectorTagsFKBP12DD (L106P)ExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbmCherry
Plasmid#184676PurposeNIR-Fb to mCherryDepositorInsertNIR-FbmCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR/pSM2(CMV) GFP (w513-1)
Plasmid#19170PurposeGateway entry vector for miRNA expression under CMV promoter and GFP reporterDepositorTypeEmpty backboneUseRNAi; Entry vectorTagsGFPExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II 31-52
Plasmid#35179DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationDeletion of C-terminal domain repeats 1-30PromoterCMVAvailable sinceOct. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2-P2A-EGFP
Plasmid#108896PurposeMultisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertAPEX2-P2A-EGFP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR/pSM2(CMV) (w57-1)
Plasmid#17389PurposeGateway entry vector for miRNA expression under CMV promoterDepositorTypeEmpty backboneUseRNAi; Entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II 1-22
Plasmid#35178DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationDeletion of C-terminal domain repeats 23-52PromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME-EGFP-P2A-APEX2-NS
Plasmid#108895PurposeMultisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertEGFP-P2A-APEX2
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceMay 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-csGBP
Plasmid#108891PurposeMultisite gateway vector for 3' tagging with a conditionally stable nanobody against GFPDepositorInsertcsGBP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B INCENP mCherry
Plasmid#45235PurposeTarget INCENP to centromeres via full length CENP-BDepositorInsertCenp-B, INCENP-Δcen, mCherry
UseTagsfull length CENP-B and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Lifeact mApple
Plasmid#187687PurposeIn vivo visualization of actinDepositorInsertLifeact
UseLentiviralTagsmAppleExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb2E7
Plasmid#184681PurposeNIR-Fb to HIV gp41DepositorInsertNIR-Fb2E7
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B INCENP GFP
Plasmid#45238PurposeTarget INCENP to centromeres via full length CENP-BDepositorInsertCenp-B, INCENP-Δcen, GFP
UseTagsGFP and full length CENP-BExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E-csChBP
Plasmid#108893PurposeMultisite gateway vector for 3' tagging with a conditionally stable nanobody against mCherryDepositorInsertdChBP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceJune 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
LacI INCENP
Plasmid#45236PurposeTarget INCENP to lac operator sequenceDepositorInsertLacI, INCENP-Δcen, mCherry (lacI )
UseTagsLacI (lac repressor protein) and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)Promotert7Available sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb4EIG
Plasmid#184683PurposeNIR-Fb to E.coli dihydrofolate reductaseDepositorInsertNIR-Fb4EIG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-NTAP-C (w324-1)
Plasmid#17404PurposeGateway entry vector for NTAP (N-terminal affinity purification), frame CDepositorTypeEmpty backboneUseEntry vectorTagsCBP and SBPExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II CC
Plasmid#35180DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationContains C-terminal domain repeats (1-22)X2PromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb59H10
Plasmid#184682PurposeNIR-Fb to HIV p24DepositorInsertNIR-Fb59H10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only