Addgene

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pWZ414-F53

Plasmid

#23077

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R

Promoter

Available since

April 12, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F30

Plasmid

#23066

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed lysine 9 to Glutamine H3 K9Q

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
KVS1-HA

Plasmid

#113061

Purpose

Expresses worm KVS-1 in mammalian cells

Depositor

Federico Sesti

Article

Cai et al Nat Neurosci. 2005 Nov;8(11):1503-9. doi

Insert

K+ voltage sensitive channel subunit 1 (kvs-1 Nematode)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL70005

Plasmid

#50335

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pET-32M human DKK1c (183–266)

Plasmid

#132626

Purpose

expresses human DKK1c (183–266) in E. coli

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

DKK1 (183–266)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmSmaug-V5His6_C

Plasmid

#146040

Purpose

Insect Expression of DmSmaug

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

DmSmaug (smg Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and a deletion of the AA 961-…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm2_A

Plasmid

#145881

Purpose

Insect Expression of DmLSm2

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm2 (CG10418 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm3_A

Plasmid

#145882

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm5_A

Plasmid

#145884

Purpose

Insect Expression of DmLSm5

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm5 (CG6610 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm7_A

Plasmid

#145885

Purpose

Insect Expression of DmLSm7

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm7 (LSm7 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJFRC7-RFPnls-PQRv2-mCD8:GFP

Plasmid

#73974

Purpose

Protein Quantitation Reporter (PQR) to quantify a protein of interest in insect cells.

Depositor

Brian Chen

Article

Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

Inserts

sfGFP
RFP

Use

Tags

mCD8 and nls

Expression

Insect

Mutation

Promoter

20XUAS-HS

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pWZ414-F31

Plasmid

#23067

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed lysine 14 to Glutamine K14Q

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmTral

Plasmid

#21722

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Trailer hitch (tral Fly)

Use

Drosophila expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pICH44300

Plasmid

#50340

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, act2 (A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTSA-38

Plasmid

#17328

Depositor

Takeshi Sano

Article

Sano et al Proc Natl Acad Sci U S A. 1995 Apr 11. 9

Insert

streptavidin

Use

Tags

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Promoter

Available since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only
pICH78133

Plasmid

#50292

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + chloroplast transit peptide, RbcS (synthetic)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pG418R-GMRWhite

Plasmid

#165883

Purpose

G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101114

Purpose

Encodes dCas9(N) fused to CXCR4

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

CXCR4-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pICH45244

Plasmid

#50277

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, RbcS3B (AT5g38410, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
M20 siamois-luciferase p01234

Plasmid

#17157

Depositor

Randall Moon

Article

Brannon et al Genes Dev. 1997 Sep 15. 11(18):2359-70.

Insert

siamois promoter (sia1.L Frog)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only