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pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172505

Purpose

Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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FH-TET3-HxDmut-pEF

Plasmid

#127896

Purpose

Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A

Depositor

Patrick Hogan, Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

TET3-HxDmut (TET3 Human)

Use

Tags

HA and Flag

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDF0039 DisCas7-11 only expression with CRISPR array

Plasmid

#172500

Purpose

Encodes the DisCas7-11 protein only for bacterial expression

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 only expression with CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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mIFP12-sEspin-C-18

Plasmid

#56262

Purpose

Localization: Actin Bundling, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

sEspin

Use

Tags

mIFP12

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Twitch-2C pcDNA3

Plasmid

#104501

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Gi-c009

Plasmid

#173079

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Use

Tags

mCitrine

Expression

Bacterial

Mutation

full length, codon optimized

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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mIFP-Lysosomes-20

Plasmid

#56228

Purpose

Localization: Lysosome Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

LAMP1

Use

Tags

mIFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
FH-TET3-CXXCmut-pEF

Plasmid

#127895

Purpose

Expresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAA

Depositor

Patrick Hogan, Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

TET3-CxxCmut (TET3 Human)

Use

Tags

HA and Flag

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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CoV-NSP3-c001

Plasmid

#173082

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep

Use

Tags

mCerulean

Expression

Bacterial

Mutation

E1000-K1173, codon optimized

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MDO2-c003

Plasmid

#173105

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD2 (MACROD2 Human)

Use

Tags

Expression

Bacterial

Mutation

K7-D243

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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MDO2-c005

Plasmid

#173106

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD2 (MACROD2 Human)

Use

Tags

Nanoluc

Expression

Bacterial

Mutation

K7-D243

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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HA-Cdk9 T186A

Plasmid

#28103

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Use

Tags

3x HA

Expression

Bacterial and Mammalian

Mutation

Contains T186A mutation in Cdk9

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pMRXIP-Myc-SNAP29

Plasmid

#116944

Purpose

Expressing Myc-SNAP29 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

synaptosomal-associated protein, 29kDa (SNAP29 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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FusionRed-VASP-5

Plasmid

#56136

Purpose

Localization: Focal Adhesions, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

VASP

Use

Tags

FusionRed

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pACYC-Cas12g1-noncoding

Plasmid

#120880

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12g1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-tdTomato

Plasmid

#153424

Purpose

Retroviral (MSCV) expression of tdTomato fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

tdTomato

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gLacZ.hSyn.flex.H2B.RFP

Plasmid

#170373

Purpose

Negative control guide, Cre-inducible plasmid expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12c2-noncoding

Plasmid

#120877

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12c2

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-DRE-1-pcDNA3.1-

Plasmid

#52513

Purpose

expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pSC44

Plasmid

#104818

Purpose

CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from AtUBQ10 promoter.

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.04g057400

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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