Addgene

We narrowed to 24,086 results for: sta

Showing: 1661 - 1680 of 24086 results

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pUdOm7.1

Plasmid

#210287

Purpose

Empty backbone with zeocin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm8.1

Plasmid

#210288

Purpose

Empty backbone with zeocin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm2.0

Plasmid

#210300

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTR

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApaLIE225A, K227A-HA

Plasmid

#211460

Purpose

Express mitochondrial localized ApaLI with E225A and K227A mutations

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-E225A-K227A

Use

Tags

HA

Expression

Mammalian

Mutation

E225A, K227A

Promoter

CMV

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEF mito-PAcGFP

Plasmid

#188905

Purpose

Expresses mitochondrial photoactivatable GFP

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

mitochondrial photoactivatable GFP

Use

Tags

Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMVS184

Plasmid

#176294

Purpose

Reporter_ZIFUAS_minCMVpromoter_GFP_AAVS1_Puromycin

Depositor

Max Staller

Article

Staller et al Cell Syst. 2022 Apr 20;13(4):334-345.e5.

Insert

Promoter and GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

4x Zinc finger binding sites and mini CMV promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod-Doc(WT)

Plasmid

#157675

Purpose

Expression of XMod-Doc wild type with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc Wild type with Histag and SpyTag

Use

Tags

Histag and SpyTag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-Coh-His-SpyTag

Plasmid

#157673

Purpose

Expression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

Cohesin (R.f) with Histag and SpyTag

Use

Tags

Histag and SpyTag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCVD402

Plasmid

#66148

Purpose

to make a probe to detect Heat stable enterotoxin gene (human)

Depositor

James Kaper

Article

Nishibuchi et al J Clin Microbiol. 1988 Apr;26(4):784-6.

Insert

STh

Use

Cloning vector

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pCVD404

Plasmid

#66149

Purpose

to make a probe to detect Heat stable enterotoxin gene (porcine)

Depositor

James Kaper

Article

Nishibuchi et al J Clin Microbiol. 1988 Apr;26(4):784-6.

Insert

STp

Use

Cloning vector

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-TetOne-Puro-GFP

Plasmid

#171123

Purpose

Doxycycline inducible expression of GFP

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pHL-sec

Plasmid

#99845

Purpose

Mammalian expression with secretion signal sequence

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EFNB2 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCasSA

Plasmid

#98211

Purpose

CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

Depositor

Quanjiang Ji

Article

Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_LifeAct-iBlueberry

Plasmid

#73176

Purpose

Expressing iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

LifeAct-iBlueberry

Use

Tags

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBMN YFP-DHFR(DD)

Plasmid

#29326

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

YFP-DHFR(DD)

Use

Retroviral

Tags

HA and YFP

Expression

Mutation

Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_HA1h

Plasmid

#190473

Purpose

Express the high-affinity histamine sensor GRAB_HA1h in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine sensor GRAB_HA1h (high affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCu-MCP-2yeGFP(405)

Plasmid

#121419

Purpose

Expressing MCP-2yeGFP

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

CUP1p-MCP-2yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1 promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_HA1m

Plasmid

#190474

Purpose

Express the medium-affinity histamine sensor GRAB_HA1m in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine sensor GRAB_HA1m (medium affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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KillerOrange /pQE-30

Plasmid

#74748

Purpose

KillerOrange gene for bacterial expression

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

Insert

KillerOrange

Use

Tags

HisTag

Expression

Bacterial

Mutation

KillerRed with substitutions G3C Y66W D113S N145S…

Promoter

T5

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_HA1mut

Plasmid

#190475

Purpose

Express the histamine-insensitive control sensor GRAB_HA1mut in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine-insensitive sensor GRAB_HA1mut (control sensor)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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