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Plasmid#26054DepositorInsertCore Traptavidin
UseTagsExpressionBacterialMutationStreptavidin with 2 amino acid mutations: S52G R5…PromoterAvailable sinceAug. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
wt HIV-1 integrase in pET15b
Plasmid#61668Purposewild type HIV-1 integrase in pET-15bDepositorInsertwild type HIV-1 integrase
UseTagsHisExpressionMutationPromoterT7 promotorAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a SpyCatcher-SnoopCatcher
Plasmid#72324PurposeBacterial expression of SpyCatcher linked to SnoopCatcher.DepositorInsertpET28a SpyCatcher-SnoopCatcher
UseTagsN-terminal His6 tagExpressionBacterialMutation35 amino acids at the N-term of SpyCatcher have b…PromoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24-ISWI-iCBD
Plasmid#105499PurposeExpression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)DepositorInsertISWI-iCBD
UseTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Trx-mSA2
Plasmid#52320PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, S tag, TEV site, and ThioredoxinExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30 SUMO-1 G97
Plasmid#13381DepositorInsertSUMO-1, G97 (SUMO1 Human)
UseTagsHisExpressionBacterialMutationG97 mutant: lacks 4 amino acids from the C-termin…PromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mTurquoise2-CNA35
Plasmid#61602Purposebacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2DepositorInsertmTurqouise and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization Control
Plasmid#186553PurposeNegative Control mediating endosomal localizationDepositorInsert10R-8His-EGFP
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b CnA CnB
Plasmid#11787DepositorUseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp7-His
Plasmid#11825DepositorInsertCaspase 7 (CASP7 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CATdeltaHD
Plasmid#174811PurposeBacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomainDepositorInsertPARP1-CATdeltaHD (PARP1 Human)
UseTags6xHisExpressionBacterialMutationResidues 678-787 replaced by eight-residue linker…PromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-mGSDMD
Plasmid#111560PurposeExpress mouse GSDMD in E.coliDepositorInsertGasdermin D (Gsdmd Mouse)
UseTagsHis, SUMOExpressionBacterialMutationPromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2
Plasmid#111086PurposeFor production of recombinant siRNAs (pro-siRNAs) in E. coli.DepositorTypeEmpty backboneUseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-mLin28A-cHIS
Plasmid#137998PurposeLin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cellsDepositorInsertmouse Lin28A, CSD and ZnFs (residues 36–186)
UseTagsC-terminal His6 tagExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet42c(+)-GST-USP13
Plasmid#61749Purposeexpress N-terminally GST tagged human USP13DepositorInsertUSP13 (USP13 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21d-myc-BirA
Plasmid#109424PurposeBacterial expression of biotin ligase (BirA) with an N-terminal myc epitopeDepositorInsertBirA (birA )
UseTagsmycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Keap1
Plasmid#62454PurposeHis6 tagged Keap1 for E.coli expressionDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-ART
Plasmid#174810PurposeBacterial expression of isolated PARP1 ART subdomainDepositorInsertPARP1-ART (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-BRCT
Plasmid#174807PurposeBacterial expression of isolated PARP1 BRCT domainDepositorInsertPARP1-BRCT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-WGR
Plasmid#174808PurposeBacterial expression of isolated PARP1 WGR domainDepositorInsertPARP1-WGR (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only