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Plasmid#221491PurposeNKX2-5 lentiviral overexpressionDepositorInsertNKX2-5 (NKX2-5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1
Plasmid#221472PurposeGateway cloning of NKX2-1 short isoform overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D3
Plasmid#204182PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertExon D3
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D1.61C>G
Plasmid#204179PurposepRegev_D1 with single C>G mutation.DepositorInsertExon D1 with 61C>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation61C>G mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D2.30C>G
Plasmid#204181PurposepRegev_D2 with single C>G mutation.DepositorInsertExon D2 with 30C>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation30C>G mutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D4.34C>G
Plasmid#204185PurposepRegev_D4 with single C>G mutation.DepositorInsertExon D4 with 34C>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation34C>G mutationPromoterTruncated CAGAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D3.48C>G
Plasmid#204183PurposepRegev_D3 with single C>G mutation.DepositorInsertExon D3 with 48C>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation48C>G mutationPromoterTruncated CAGAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1647
Plasmid#155148PurposeExpresses MlaF 1-246, taglessDepositorInsertmlaF(1-246)
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1840
Plasmid#155150PurposeExpresses MlaF-6xHisDepositorInsertMlaF-6xHis
UseTagsExpressionMutationPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1266
Plasmid#155142PurposeExpresses MlaF, tagless, araBAD promoterDepositorInsertMlaF
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) short BP-3'SS
Plasmid#111333PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationshorter branch to 3' splice site distancePromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc M1
Plasmid#106321PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc mutated splice site EEKQ-->AAAAPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc M5
Plasmid#106325PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc splice site insertion mutant EEKQNA-->EEK…PromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc M4
Plasmid#106324PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc splice site deletion mutant EEKQNA-->EEKNAPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc M3
Plasmid#106323PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc mutated splice site EEKQNAV-->AAAAAAAPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-HSE1-iGFPx6
Plasmid#105259PurposePlasmid for pop-in/pop-out replacement of Hse1 with Hse1-iGFPx6.DepositorInsertHse1 portion (HSE1 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-MUP1-iYFP
Plasmid#105266PurposePlasmid for pop-in/pop-out replacement of Mup1 with Mup1-iYFP.DepositorInsertMup1 portion (MUP1 Budding Yeast)
UseTagsiYFPExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a1-368.E152Q
Plasmid#17517DepositorInsertB. subtilis DEAD-box helicase YxiN first two domains
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 369-479; E152Q mutat…PromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
MERTK_HUMAN_D0
Plasmid#79705PurposeThis plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMERTK (MERTK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCC2G_HUMAN_D0
Plasmid#79711PurposeThis plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2G (CAMK2G Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only