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pSWAP_Entry_HB

Plasmid

#184868

Purpose

pSwap plasmid part with HB homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pRL277

Plasmid

#70255

Purpose

sacB plasmid with polylinker; streptomycin/spectinomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

Depositor

Peter Wolk

Article

Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

Type

Empty backbone

Use

Conditionally lethal

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRO16Sh2

Plasmid

#180604

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLac

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAC1823_pCR8-RBFOX1N-dCasRx-C

Plasmid

#118657

Purpose

Gateway Donor; RBFOX1N-dCasRx-C ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBFOX1N-dCasRx-C

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

No promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAF1-VP16

Plasmid

#51426

Purpose

Artificial transcription factor with VP16

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pSNAP-macroH2A1.2 Zeo

Plasmid

#132428

Purpose

Expressing SNAP-tagged histone macroH2A1.2 in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

macroH2A1.2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p155 pCMV-EGFP/beta-gal

Plasmid

#8387

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox; Beta-gal

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pTOX1

Plasmid

#127446

Purpose

Encodes YhaV toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

yhaV toxin

Use

Tags

Expression

Bacterial

Mutation

Promoter

pRha

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-fullef1a-FAKSPARK2

Plasmid

#195265

Purpose

express wtFAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

FAKSPARK2 sensor

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

ef1a

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2G1

Plasmid

#15790

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
EasyFusion eGFP

Plasmid

#112848

Purpose

General donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

EGFP

Use

Unspecified; General donor

Tags

Expression

Mutation

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCJH021_SC101_pLAC_Cas12c2_Kan

Plasmid

#183072

Purpose

Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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HO-pGAL-poly-KanMX4-HO

Plasmid

#51664

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-Vasa-GFP

Plasmid

#135595

Purpose

Expression of Vasa-GFP mRNA for microinjection for visualization of Vasa protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Yajima et al Development. 2015 Jun 1;142(11):1960-70.

Insert

Vasa-GFP

Use

In vitro transcription (mrna synthesis)

Tags

EGFP

Expression

Mutation

Promoter

SP6

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEA106

Plasmid

#187872

Purpose

Expresses mCherry under the virB promoter.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Agrobacterium virB promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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p133 pSV-STOP-luc

Plasmid

#8390

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed STOP

Use

Cre/Lox and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pKF295

Plasmid

#72871

Purpose

expression of improved FLP recombinase

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

FLP recombinase (flp Budding Yeast)

Use

Tags

Expression

Insect

Mutation

P2->S, added NLS

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAC1825_pCR8-dCasRx-RBM38

Plasmid

#118662

Purpose

Gateway Donor; dCasRx-RBM38 ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-RBM38

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

No promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pdhfr

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSC201plus

Plasmid

#164227

Purpose

Integrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression

Depositor

Silvia Cardona

Article

Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Rhamnose-inducible

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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