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pBabe puro pI3K p110 CAAX

Plasmid

#13339

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

pI3K p110 CAAX (PIK3CA Bovine)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only
3349 pSFFV-neo Bcl-2

Plasmid

#8769

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
HA-AMOT p130 deltaCC

Plasmid

#32827

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

E5D, G858D, deletion of amino acids 410-751

Promoter

CMV

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pYFAC

Plasmid

#168982

Purpose

Filamentous fungi vector. Also referred to as pYFAC-pyrG.

Depositor

Yit Heng Chooi

Article

Chooi et al Environ Microbiol. 2017 May;19(5):1975-1

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Codon Optimized Dysferlin

Plasmid

#67878

Purpose

The human dysferlin cDNA was codon optimized in an attempt to increase Dysferlin production in human cells.

Depositor

Matthew Hirsch

Article

Hirsch et al Mol Ther. 2013 Dec;21(12):2205-16. doi:

Insert

codon optimized Dysferlin

Use

Unspecified

Tags

No

Expression

Mutation

Promoter

No Promoter

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE8

Plasmid

#79463

Purpose

sgRNA shuttle vector; module 2E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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P303-FBP_2

Plasmid

#139693

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TTRmiRc2

Plasmid

#25754

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

dsRed2

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiRc3

Plasmid

#25749

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pET22-HT/prsA

Plasmid

#73624

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

Depositor

James Williamson, T. J. Kappock

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_3xFlagEzh2 RNA binding mutant

Plasmid

#173718

Purpose

RNA-binding mutant EZH2 expression plasmid with 3XFLAG tag

Depositor

Thomas Cech

Article

Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

Insert

EZH2 RNA-binding mutant

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-krt4-GFP

Plasmid

#97151

Purpose

label apical epithelial cells in zebrafish

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

AcGFP

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE6

Plasmid

#79476

Purpose

sgRNA shuttle vector; module 1E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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mPERK

Plasmid

#24826

Depositor

Fumihiko Urano

Article

Ghosh et al PLoS One. 2010 . 5(3):e9575.

Insert

mPERK (Eif2ak3 Mouse)

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 S454A

Plasmid

#19844

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 S454A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 454 to Alanine

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
c-myc promoter (4x TBE2 - wt)

Plasmid

#16599

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE2 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pRSV-p65S276A

Plasmid

#106455

Purpose

NF-kB p65, kinase site 276 mutated

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65, kinase site 276 mutated (RELA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

kinase site inactivated

Promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pconst #1-RFP

Plasmid

#81075

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter - BBa_J23119

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe hygro myc p110-CAAX

Plasmid

#12591

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

p110-CAAX (PIK3CA Bovine)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only