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pAS1_4x7SKPylT_EF1_Ub-BsmBI

Plasmid

#162801

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0113

Plasmid

#117552

Purpose

Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCALNL-BsaI-EGFP

Plasmid

#123136

Purpose

Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

EGFP, mRFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

cBA

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGGC001

Plasmid

#48824

Purpose

Provides WUS as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS (WUS Mustard Weed)

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

internal BsaI recognition site removed by silent …

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBT195

Plasmid

#127843

Purpose

clone BE4max CBEs by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max without deaminase

Use

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTU2_2a

Plasmid

#73010

Purpose

Secondary module (BpiI) site in pTU2a backbone

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Secondary module + Golden Gate pTU2a BsmBI destination site

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFUS_A8

Plasmid

#99882

Purpose

Recipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0011

Plasmid

#117530

Purpose

Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-xCas9 3.7 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pR-mT-Sapphire

Plasmid

#228597

Purpose

Mammalian expression of mT-Sapphire, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mT-Sapphire

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mAmetrine

Plasmid

#228598

Purpose

Mammalian expression of mAmetrine, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mAmetrine

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mCerulean3

Plasmid

#228599

Purpose

Mammalian expression of mCerulean3, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mCerulean3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pR-EGFP

Plasmid

#228603

Purpose

Mammalian expression of EGFP, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mClover3

Plasmid

#228605

Purpose

Mammalian expression of mClover3, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mClover3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mVenus

Plasmid

#228606

Purpose

Mammalian expression of mVenus, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mPapaya

Plasmid

#228607

Purpose

Mammalian expression of mPapaya, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mPapaya

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mCardinal

Plasmid

#228611

Purpose

Mammalian expression of mCardinal, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mCardinal

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only
pM-EBFP2

Plasmid

#228614

Purpose

Mammalian expression of EBFP2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

EBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mT-Sapphire

Plasmid

#228616

Purpose

Mammalian expression of mT-Sapphire and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mT-Sapphire

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mAmetrine

Plasmid

#228617

Purpose

Mammalian expression of mAmetrine and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mAmetrine

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mCerulean3

Plasmid

#228618

Purpose

Mammalian expression of mCerulean3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mCerulean3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge