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Plasmid#39160PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHSD17B14 (HSD17B14 Human)
UseTagsHis TevExpressionBacterialMutationcodon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIA036
Plasmid#200425PurposeamyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorArticleInsertLacI
UseTagsbsdronPAExpressionBacterialMutationPromoterPpenAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-PAG1-GFP
Plasmid#198223PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertPAG1 (PAG1 Human)
UseLentiviralTagsFollowed by EGFP, after a flexible linker and Pre…ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LZ3 dFE
Plasmid#190144PurposeExpresses LZ3Cas9-T4pol (LZ3 dFE)DepositorInsertLZ3Cas9-T4pol (LZ3 dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiFi dFE
Plasmid#190142PurposeExpresses HiFiCas9-T4pol (HiFi dFE)DepositorInsertHiFi Cas9-T4pol (HiFi dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationSpCas9 R691A mutant, Codon Optimized for mammalia…PromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1
Plasmid#160448PurposeBioBrick mammalian-codon-optimized mRFP1DepositorInsertpromoter, RBS, mRFP1
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-dsRed
Plasmid#160449PurposeBioBrick mammalian-codon-optimized dsRedDepositorInsertpromoter, RBS, dsRed
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134186
Plasmid#152962PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TapTagExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133251
Plasmid#152818PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133367
Plasmid#152791PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;S-tagExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133558
Plasmid#152777PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TRXExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133345
Plasmid#152768PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TapTagExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133650
Plasmid#152746PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TRXExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4134219
Plasmid#152544PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TapTagExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4134210
Plasmid#152521PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;CBPExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133876
Plasmid#152450PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;S-tagExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133627
Plasmid#152376PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133623
Plasmid#152320PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TRXExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133264
Plasmid#152137PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;S-tagExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133354
Plasmid#152134PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits