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Plasmid#202509PurposeExpress 40 Angstrom 4TMD de novo proteinDepositorInsert40 Angstrom 4TMD de novo
UseTagsmRFP1-3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-roxSTOProx-tRFP-pA-lox
Plasmid#196888PurposeDre-activated Cre-inactivated expression of turbo (t)RFP reporter.DepositorInsertturbo (t)RFP
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-miRFP713-RfA
Plasmid#186381PurposeDestination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2zmiRFP-EF1-2xCMV-EF1-mClover3
Plasmid#196112Purposemammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3DepositorInsertzmiRFP-EF1-2xCMV-EF1-mClover3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3
Plasmid#196111Purposemammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3DepositorInsertzmiRFP2-EF1-2xCMV-EF1-mClover3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miRFP670-2-P2A-GFP
Plasmid#197170PurposeExpresses the protein of miRFP670-2-P2A-GFP in mammalian cellsDepositorInsertmiRFP670-2-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iRFP670-P2A-GFP
Plasmid#197169PurposeExpresses the protein of iRFP670-P2A-GFP in mammalian cellsDepositorInsertiRFP670-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iRFP713-P2A-GFP
Plasmid#197166PurposeExpresses the protein of iRFP713-P2A-GFP in mammalian cellsDepositorInsertiRFP713-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-emiRFP703-P2A-GFP
Plasmid#197158PurposeExpresses the protein of emiRFP703-P2A-GFP in mammalian cellsDepositorInsertemiRFP703-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN-CAG-mRFP-PCNA pc2729
Plasmid#166040PurposeExpresses mRFP tagged human PCNA in mammalian cells.DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3
Plasmid#196109Purposemammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3DepositorInsertzTagRFP658-EF1-2xCMV-EF1-mClover3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2zemiRFP2-EF1-2xCMV-EF1-mClover3
Plasmid#196110Purposemammalian/zebrafish expression of zemiRFP2-EF1-2xCMV-EF1-mClover3DepositorInsertzemiRFP2-EF1-2xCMV-EF1-mClover3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)
Plasmid#186703PurposeMammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)DepositorInsertmiRFP703-eDHFR(69K6)-Tiam1(DH-PH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-miRFP713-stop
Plasmid#186391PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[HA]_IRES_iRFP670-H2A-P2A-PuroR
Plasmid#190080Purposeexpress cytoplasmically HA-tagged human ACE2 with selectable markers iRFP670 and puromycin resistance geneDepositorInsertACE2 (ACE2 Human)
UseTagsHAExpressionMammalianMutationPromoternoneAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-RFP_mmu-miR-292b-3p_sponge
Plasmid#132623PurposeExpresses a TurboRFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA spongesDepositorInsertstop codon, mmu-miR-292b-3p sponge sequence and polyA signal
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chGrb2/eDHFR(69K6)-iRFP713
Plasmid#178854PurposeMammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713DepositorInsertGrb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
UseTagsExpressionMammalianMutationGrb2: Y160EPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670
Plasmid#178858PurposeMammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670DepositorInsertPKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s
Plasmid#172105PurposeMammalian expression of Gαs fused to miRFP670-eDHFR(69K6)DepositorInsertmiRFP670-eDHFR(69K6)-Gαs (GNAS Human)
UseTagsExpressionMammalianMutationGαs: C3SPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-rScn2a-3xV5-Syn-miRFP670
Plasmid#182566PurposeFor rat Nav1.2 knockin with 3xV5 at the extracellular loopDepositorInsertsrat Scn2a gRNA and 3xV5 donor DNA
miRFP670
UseAAV and CRISPRTagsExpressionMutationPromoterSyn and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only