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H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)

Plasmid

#189963

Purpose

Coexpression of CBP TAZ1 and CITED2 in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-269) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

L243A, E245A, L246A

Promoter

t7

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)

Plasmid

#189962

Purpose

Expression of CBP TAZ1 and CITED2 in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-246) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

t7

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ILDR1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156537

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ILDR1 (ILDR1 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-712pUC19-tNGFR-P2A-ITCH

Plasmid

#186085

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

ITCH (ITCH Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFbxw7#2/Cre

Plasmid

#173636

Purpose

Expresses a Fbxw7-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Fbxw7 (Fbxw7 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9-CBP RING-PHD-HAT

Plasmid

#179549

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) (CREBBP Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9-CBP PHD-HAT

Plasmid

#179548

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) (CREBBP Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HOXB3

Plasmid

#101478

Purpose

Donor Vector containing HOXB3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXB3 (HOXB3 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A_mmFbxw7_R505C (closed)

Plasmid

#160103

Purpose

Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7 alpha R505C (Fbxw7 Mouse)

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

R505C

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYLC2_p.D299Y

Plasmid

#81608

Purpose

Gateway Donor vector containing CYLC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYLC2 (CYLC2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D299Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2IP_p.I979M

Plasmid

#81568

Purpose

Gateway Donor vector containing ERBB2IP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2IP (ERBIN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

T271P; D547G; I979M; E1205G; Q1226P; K1287R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYLC2_p.V105F

Plasmid

#81392

Purpose

Gateway Donor vector containing CYLC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYLC2 (CYLC2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

V105F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYLC2_p.E133K

Plasmid

#81385

Purpose

Gateway Donor vector containing CYLC2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYLC2 (CYLC2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E133K; N334K; A335G; del AA 337-348

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYLC2_p.E152K

Plasmid

#81388

Purpose

Gateway Donor vector containing CYLC2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYLC2 (CYLC2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E152K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYLC2_p.R71C

Plasmid

#81362

Purpose

Gateway Donor vector containing CYLC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYLC2 (CYLC2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R71C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ISX_p.R86C

Plasmid

#81365

Purpose

Gateway Donor vector containing ISX , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ISX (ISX Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S28G; P57S; R86C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ISX_p.T87A

Plasmid

#81323

Purpose

Gateway Donor vector containing ISX , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ISX (ISX Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S28G, P57S, T87A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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zgc:162148_L (OZ579)

Plasmid

#35219

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:162148 (micu1 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223_FBXW7_WT

Plasmid

#81795

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-JAG1-Fc(DAPA)-AviTag-6xHis

Plasmid

#157024

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

JAG1 (JAG1 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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