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Plasmid#136955PurposePlasmid for the In Vitro transcription of DiySpike2d1DepositorInsertDiySpike2d1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike2d2
Plasmid#136956PurposePlasmid for the In Vitro transcription of DiySpike2d2DepositorInsertDiySpike2d2
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike2d3
Plasmid#136957PurposePlasmid for the In Vitro transcription of DiySpike2d3DepositorInsertDiySpike2d3
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike2d4
Plasmid#136958PurposePlasmid for the In Vitro transcription of DiySpike2d4DepositorInsertDiySpike2d4
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISL1.1.0-gDNA
Plasmid#113788PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ISL1DepositorInsertISL1 (ISL1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.zeo/hKGA.S286A
Plasmid#110357PurposeMammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tagDepositorInsertKGA glutaminase (GLS Human)
UseRetroviralTagsV5ExpressionMammalianMutationS286APromotergagAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 K659M 3xFlag
Plasmid#110112Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Lysine 659 to Methiionine (K659M)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 E565A 3xFlag
Plasmid#110109Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Glutamic acid 565 to Alanine (E565A)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 K641R 3xFlag
Plasmid#110111Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Lysine 641 to Arginine (K641R)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 N549H 3xFlag
Plasmid#110106Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Asparagine 549 to Histidine (N549H)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 N549K 3xFlag
Plasmid#110107Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Asparagine 549 to Lysine (N549K)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 L617V 3xFlag
Plasmid#110110Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Leucine 617 to Valine (L617V)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
C21orf88_pCSdest
Plasmid#53950DepositorInsertC21orf88 (B3GALT5-AS1 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceSept. 8, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
GNE
Plasmid#25255PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGNE:T406-R720 (GNE Human)
UseTagsHisExpressionBacterialMutationContains amino acids T406-R720PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX2TK E7 del EDE
Plasmid#13675DepositorInsertHPV 16 E7 del EDE (E7 Human)
UseTagsGSTExpressionBacterialMutationdel aa 35-37PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMarVF1
Plasmid#99579PurposeMinitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114DepositorInsertV. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
UseSuicide vector in v. fischeri es114TagsExpressionMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAV1-mEGFP
Plasmid#27704DepositorInsertcaveolin-1 (CAV1 Canis lupus familiaris)
UseTagsmonomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-H2B-mCherry
Plasmid#89766PurposeLentivirus destination vector for stable expression of mCherry-tagged histone H2BDepositorInsertHistone H2B (H2BC11 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_hNMT_Nef
Plasmid#66078PurposeSingle vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertsUseTagsExpressionBacterialMutationDeletion of first 80 amino acidsPromoterT7-lacOAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
p11d-sctRPA
Plasmid#102614PurposeExpression of S. cerevisiea RPA in E.coliDepositorUseTagsExpressionBacterialMutationRFA2 has intron deletedPromoterT7Available sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only