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Plasmid#154762Purposeplasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEC819
Plasmid#190815PurposeCRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorInsertdCas9-XTEN-rpoA
UseCRISPR and Synthetic BiologyTagsXTEN (linker), followed by the N-terminal domain …ExpressionMutationPromoterSP30Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
HDAC4 Flag
Plasmid#13821PurposeMammalian expression of human histone deacetylase 4 with flag tagDepositorInsertHDAC4 (HDAC4 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS159
Plasmid#122378PurposeCRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10 Trp S. cerevisiae expression vector (12TRP-U)
Plasmid#48303DepositorTypeEmpty backboneUseNaTagsExpressionMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
psfTn5
Plasmid#79107Purposebacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutationsDepositorInserttransposase 5
UseTagsHisExpressionBacterialMutationE54K and L372PPromoterAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-BioID2-BioID2-HA
Plasmid#176740PurposeExpresses the promiscuous small biotin ligase, BioID2, in astrocytesDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GRIK1_pCSdest
Plasmid#53832DepositorInsertGRIK1 (GRIK1 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLyGo-Kp-1
Plasmid#163143PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAOX1Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10 Ura S. cerevisiae expression vector (12URA-U)
Plasmid#48306DepositorTypeEmpty backboneUseNaTagsExpressionMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only