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pEMS1174

Plasmid

#29088

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple240 (UGT8 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1430

Plasmid

#29225

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple170 (POGZ Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(RK)

Plasmid

#27136

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Arginines in the SH2 domains mutated to lysines (…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(FF)

Plasmid

#27135

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Tyrosines in ITAMS 1-3 mutated to phenylalanines …

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1215

Plasmid

#29109

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple97 (GPX3 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pVenusNfN

Plasmid

#128564

Purpose

Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

Depositor

Oliver Vugrek

Article

Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

Insert

Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pVenusCfC

Plasmid

#128567

Purpose

Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

Depositor

Oliver Vugrek

Article

Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

Insert

Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pVenusCfN

Plasmid

#128566

Purpose

Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

Depositor

Oliver Vugrek

Article

Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

Insert

Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAB-HDAC5(mut-H-Y)

Plasmid

#114398

Purpose

For PYL-HDAC5 H1006Y expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 H1006Y

Use

Tags

Expression

Mammalian

Mutation

additional D593E compared to NCBI ref NP_005465.2

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-myrAkt

Plasmid

#49267

Purpose

retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence

Depositor

Lawrence Kane, Arthur Weiss

Article

Kane et al Nat Immunol. 2001 Jan;2(1):37-44.

Insert

Akt (AKT1 Bovine)

Use

Retroviral

Tags

IRES-EGFP and myr

Expression

Mammalian

Mutation

N2D, D149E and A478G compared to AAW71957.1

Promoter

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pAB(EXPR-PYL-HDAC5-NEO)

Plasmid

#114395

Purpose

For PYL-HDAC5 expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

D593E compared with NCBI reference NP_005465.2

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAB-HDAC5(mut-H-A)

Plasmid

#114397

Purpose

For PYL-HDAC5 H1006A expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 H1006A

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

D593E compared to NCBI ref NP_005465.2

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAB-EXPR(PYL-Nterm_dom_HDAC5)

Plasmid

#114402

Purpose

For PYL-HDAC5 N terminal domain expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 N terminal domain

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

D593E compared to NCBI ref NP_005465.2

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pB PUb-integrase

Plasmid

#183966

Purpose

piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

PhiC31 integrase

Use

Piggybac insect transgenesis destination vector

Tags

Expression

Mutation

Promoter

Aedes aegypti Polyubiquitin

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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CMV::SypHy A4

Plasmid

#24478

Depositor

Leon Lagnado

Article

Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

Insert

SypHy (Syp Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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FHUG+W (B2)

Plasmid

#74011

Purpose

lentiviral expression of EGFP and shRNA (from H1 promoter, empty)

Depositor

Oliver Schluter, Robert Malenka, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_003

Plasmid

#192505

Purpose

For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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ER-mNeonGreen

Plasmid

#137804

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

KDEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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CMV SNAP-Synapsin

Plasmid

#206152

Purpose

CMV SNAP-Synapsin

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

Synapsin

Use

Tags

SNAPf

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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ER-mScarletI

Plasmid

#137805

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

KDEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge