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pEY67

Plasmid

#191064

Purpose

ceh-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ceh-1 (ceh-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY69

Plasmid

#191066

Purpose

ceh-24 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ceh-24 (ceh-24 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target

Plasmid

#159842

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI Pmlc-2_GFP target

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFA-DAB2

Plasmid

#162690

Purpose

Expresses DabAB2 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB2 inorganic carbon transport complex

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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KG#901

Plasmid

#110936

Purpose

Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

Inserts

mig-13 promoter
Emerald-RAB-3
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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KG#920

Plasmid

#110937

Purpose

Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

Inserts

mig-13 promoter
INS-22-Emerald
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA-DAB1

Plasmid

#162691

Purpose

Expresses DabAB1 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB1 inorganic carbon transport complex

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP

Plasmid

#159836

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI Pmlc-2_GFP

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 C539A

Plasmid

#133008

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Use

Tags

Expression

Bacterial

Mutation

C539A

Promoter

tetR

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 H524A

Plasmid

#133007

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Use

Tags

Expression

Bacterial

Mutation

H524A

Promoter

tetR

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 D353A

Plasmid

#133006

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Use

Tags

Expression

Bacterial

Mutation

D353A

Promoter

tetR

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1400

Plasmid

#78190

Purpose

Ce hsp-16.1 expression in worms

Depositor

Junho Lee

Article

Lee et al J Biol Chem. 2013 Jan 18;288(3):1582-9.

Insert

hsp-16.1 (hsp-16.1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

hsp-16.1

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pClneoEGFPC-let60 K16N

Plasmid

#66861

Purpose

Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

Let-60 (let-60 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lysine 16 is mutated to asparagine

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
AP303-4

Plasmid

#66088

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs1

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
AP334-5

Plasmid

#66096

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
APCSD 54

Plasmid

#66100

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for CSD 54

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
APCSD 53

Plasmid

#66099

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for CSD 53

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP334-6

Plasmid

#66097

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP334-2

Plasmid

#66095

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP324-4

Plasmid

#66094

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only