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sABE v3.22 Lenti_2

Plasmid

#209556

Purpose

pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

SpCas9 sgRNA, TadA-8e (2-76)-2xFRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

pU6, pCMV

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)GαoA-91-NLuc

Plasmid

#207151

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAO

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position L(91)G

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)GαoB-91-NLuc

Plasmid

#207152

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAO

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position L(91)G

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gγ1-LSS-mKATE2(N)

Plasmid

#207159

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNA

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNG1

Use

Tags

Expression

Mammalian

Mutation

N-terminally tagged with LSS-mKATE2

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pKE011

Plasmid

#207912

Purpose

Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCS018

Plasmid

#207911

Purpose

Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCS017

Plasmid

#207909

Purpose

Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCS020

Plasmid

#207915

Purpose

Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKE012

Plasmid

#207914

Purpose

Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKE010

Plasmid

#207910

Purpose

Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS019

Plasmid

#207913

Purpose

Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-4 (b)

Plasmid

#204435

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

I735A

Promoter

CMV

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET-GFP-mSOX17

Plasmid

#206379

Purpose

Expresses EGFP fused mouse SOX17 in bacteria, for protein production.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mSox17-3xflag

Plasmid

#206377

Purpose

Expresses 3xflag fused mouse SOX17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-GFP-mSox17

Plasmid

#206373

Purpose

Expresses EGFP fused mouse SOX17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-GFP-mSox17FNV

Plasmid

#206372

Purpose

Expresses EGFP fused mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-mSox17FNVC2

Plasmid

#206370

Purpose

Expresses mouse SOX17FNVC2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNVC2 (Sox17 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-mSox17FNV

Plasmid

#206368

Purpose

Expresses mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-mSox17

Plasmid

#206367

Purpose

Expresses mouse SOX17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge