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Plasmid#157501PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertCEACAM5 (CEACAM5 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFSF12-COMP5AP-AviTag-9xHis
Plasmid#157187PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNFSF12 (TNFSF12 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-MICA-COMP5AP-AviTag-9xHis
Plasmid#157289PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertMICA (MICA Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTT3-SP-6xHis-PVR(FL)-FLAG
Plasmid#157622PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorInsertPVR (PVR Human)
UseTags6xHis and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ROBO4-Fc(DAPA)-AviTag-6xHis
Plasmid#156864PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertROBO4 (ROBO4 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
GSK3B gRNA (BRDN0001145264)
Plasmid#77281Purpose3rd generation lentiviral gRNA plasmid targeting human GSK3BDepositorInsertGSK3B (GSK3B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GSK3B gRNA (BRDN0001146390)
Plasmid#77282Purpose3rd generation lentiviral gRNA plasmid targeting human GSK3BDepositorInsertGSK3B (GSK3B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FRB-GFP-Gab2(Y604F/Y634F)
Plasmid#188659PurposeFRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to PheDepositorInsertGRB associated binding protein 2 (Gab2 Mouse)
UseTagsFRB and GFPExpressionMammalianMutationY604F and Y634FPromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode
Plasmid#170254PurposeBarcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertSTAT3 (STAT3 Human)
UseLentiviralTagsExpressionMutationA662C, N664C, V667LPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ROBO1-COMP5AP-AviTag-9xHis
Plasmid#157562PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertROBO1 (ROBO1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-HLA-E-COMP5AP-AviTag-9xHis
Plasmid#157396PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertHLA-E (HLA-E Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-VSTM2L-Fc(DAPA)-AviTag-6xHis
Plasmid#156582PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertVSTM2L (VSTM2L Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-VCAM1-COMP5AP-AviTag-9xHis
Plasmid#157506PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertVCAM1 (VCAM1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-CXXC/LLAA
Plasmid#124399PurposeFull-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
UseTagsFlagExpressionMammalianMutationCXXC zinc finger deletion (aa 585-624) and L897A/…PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF655
Plasmid#88578PurposeDonor Vector containing ZNF655 transcription factor, part of the Human TFome CollectionDepositorInsertZNF655 (ZNF655 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Nr4a1sgRNA1
Plasmid#160945PurposeGuide RNA 1 to generate Nr4a1 knockout by CRISPRDepositorInsertNr4a1 gRNA 1 (Nr4a1 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTT3-SP-6xHis-KIR2DL1(FL)-FLAG
Plasmid#157625PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorInsertKIR2DL1 (KIR2DL1 Human)
UseTags6xHis and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF6B-COMP5AP-AviTag-9xHis
Plasmid#157277PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertTNFRSF6B (TNFRSF6B Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC1(Trunc1)-COMP5AP-AviTag-9xHis
Plasmid#157610PurposeMammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertSIGLEC1 (SIGLEC1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p029 End-1::EnR
Plasmid#11029DepositorUseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationDominant inhibitory GATA-binding protein End1::En…PromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only