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OTUB1
Plasmid#25149PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertotub1.040.271 (OTUB1 Human)
UseTagsHisExpressionBacterialMutationamino acid residues 40-271PromoterAvailable sinceAug. 6, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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5C8H
Plasmid#69969PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertORC2:JMC095-D11:C231601 (ORC2 Human)
UseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
UEV3
Plasmid#25621PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUEV3 (UEVLD Human)
UseTagsHisExpressionBacterialMutationL-lactate Dehydrogenase Domain, residues 171-471PromoterAvailable sinceAug. 31, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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4LD6
Plasmid#60088PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPWWP2B:JMC079-A09:C228600 (PWWP2B Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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LaminB1 (3MOV)
Plasmid#28108PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLaminB1:JMC01X-B01:C209772
UseTagsHis tag with TEV siteExpressionBacterialMutationcontains amino acid residues 311-388PromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
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SARS2_HisSUMOTEV-nsp7/nsp8
Plasmid#234405PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertsNsp7_SARS2
Nsp8_SARS2
UseTagsHis6-SUMO-TEVExpressionBacterialMutationPromoterT7Available sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_6IYE
Plasmid#234237PurposeHeterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coliDepositorInsert6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_4IPC
Plasmid#234229PurposeHeterologous protein expression of replication protein A 70 kDa DNA-binding subunit in Escherichia coliDepositorInsert4IPC (RPA1 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3CX2
Plasmid#234221PurposeHeterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coliDepositorInsert3CX2 (MYBPC3 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1KQX
Plasmid#234181PurposeHeterologous protein expression of cellular retinol-binding protein type II in Escherichia coliDepositorInsert1KQX (rbp2a Zebrafish)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_4NFB
Plasmid#234161PurposeHeterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coliDepositorInsert4NFB (PILRA Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5EF9
Plasmid#234154PurposeHeterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coliDepositorInsert5EF9 (PB2 Influenza B virus (B/Lee/1940))
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP1
Plasmid#233685PurposeEngineered SUMO protease expression plasmid designed for efficient protease-mediated elution.DepositorInsertUlp1-like SUMO protease
UseTagsCAHS-107838 and HisTagExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
PRPF4.211.522.gst
Plasmid#137650PurposeExpresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.DepositorInsertPRPF4 (PRPF4 Human)
UseTagsGST-(His)6-thrombinExpressionBacterialMutation211-522-gstPromoterAvailable sinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D12W/2Y
Plasmid#124275PurposeStudy of protein insertion into nanodisks by means of changes in tryptophan fluorescenceDepositorInsertMembrane scaffold protein
UseTags7xHis-tagExpressionBacterialMutationTwo tryptophans changed to two tyrosinesPromoterT7Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pmSod1-Q42L;Q123A-N’His
Plasmid#117710Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationQ42L; Q123APromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK611
Plasmid#72532PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)DepositorInsertsformylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsExpressionBacterialMutationPromoterT7 and n/aAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK626
Plasmid#72461PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationMutation changes serine-57 to cysteine, tyrosine-…PromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK691
Plasmid#72381PurposeProduces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)DepositorInsertstationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only
