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Plasmid#85812PurposeTo rescue the expression of DHHC5 in the knockdown cell linesDepositorInsertZDHHC5 (Zdhhc5 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNatB (pACYCduet-naa20-naa25)
Plasmid#53613PurposeAllows expression of the fission yeast NatB complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PC-GCaMP6f
Plasmid#73503PurposeGCaMP6f knock in construct into the AAVS1 locusDepositorInsertGCaMP6f
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEP9 MLH1
Plasmid#16458DepositorInsertMLH1 (MLH1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-post-mGRASP-2A-dTomato
Plasmid#34912PurposepostDepositorInsertpostsynaptic mGRASP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-ASCL1-DLX2
Plasmid#182307PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expressionUseTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and pTRE3G-bidirectionalAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
gag-P3-pol
Plasmid#211374PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertMMLVgag-P3-pol
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psMEK1tight
Plasmid#89362PurposeA lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cellsDepositorInsertpsMEK1tight (MAP2K1 Human, Synthetic)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-BI30
Plasmid#183749PurposeAAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.DepositorInsertAAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
UseAAVTagsExpressionMutationK449R, and NNSTRGG inserted between 588 and 589 o…Promoterp41Available sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIH-TFAM-GFP
Plasmid#113704PurposeFluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selectionDepositorInsertTFAM (Tfam Mouse)
UseRetroviralTagseGFPExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PCP-T5Exo
Plasmid#214736Purposeencodes T5 exonuclease with a PP7 coat protein fused to the N terminusDepositorInsertCAG_PCP-T5Exo_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
P4-PE
Plasmid#211375PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertP4-PE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgRNA
Plasmid#124844PurposeVector for Cre-dependent expression of SaCas9DepositorInsertSaCas9, gRNA scaffold
UseAAV, CRISPR, and Mouse TargetingTagsNLS and NLS-3xHAExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1
Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_TNF-SBP-mCherry
Plasmid#65279Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFMRPiso1
Plasmid#48690PurposeExpresses hFMRP iso 1DepositorInsertFlagHA FMR1 Iso 1 (FMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6c-V106W
Plasmid#215826PurposeExpress CBE6c V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6c V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lamp1_mStayGold
Plasmid#221902PurposeExpresses mStayGold fused to Lamp1DepositorInsertLamp1_mStayGold
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-V5-AP-nes_pLX304
Plasmid#120912PurposeLentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosolDepositorInsertFKBP-V5-AP-NES
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-BBz
Plasmid#135992PurposeModular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only