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Addgene
Showing: 8261 - 8270 of 152839 results

  4. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Daniel Geschwind
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201999-plasmid-map-sequence-id-398468

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  7. 3xFLAG-dCas9/pMSCVhyg

    Plasmid
    #134323
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134323-plasmid-map-sequence-id-430622

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  9. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

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Showing: 8261 - 8270 of 152839 results