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Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
CN2256-rAAV-eHGT_462m-minBglobin-SYFP2-WPRE3-BGHpA
Plasmid#164456PurposeDirect-expressing SYFP2 AAV VirusInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBetaGlobinAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-PdCO-mScarlet-WPRE
Plasmid#198510PurposeExpresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor.DepositorHas ServiceAAV5InsertPdCO
UseAAVTagsRho1D4 and mScarletExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9
Plasmid#184464PurposePlasmid encoding PRDM9-Cas9 fusion under CMV promoterDepositorInsertPRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
BIC-Gag-CAS9
Plasmid#119942Purposeencodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.DepositorInsertGAG FMLV fused to spCAS9
UseTagsFLAGExpressionMammalianMutationPromoterhCMVAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL4B-FLAG
Plasmid#15822DepositorInsertCullin4B (CUL4B Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N594PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5RGDPK7
Plasmid#198291PurposeProduces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)DepositorInsertPVCpnf_pvc13-Ad5RGDPK7
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-microID
Plasmid#172881Purposeexpresses microID in E. coliDepositorInsertmicroID
UseTagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TetON-Sox2
Plasmid#110280PurposeDoxycycline-inducible overexpression of Sox2DepositorInsertSRY (sex determining region Y)-box 2 (Sox2 Mouse)
UseLentiviralTagsNo fusion genesExpressionMammalianMutationWild typePromoterTREAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only