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DVC_p15A_FG
Plasmid#217590PurposeDestination vector with chloramphenicol resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-His-MBP-DDX3X(WT)
Plasmid#219988PurposeDDX3X(WT) protein purificationDepositorInsertDDX3X(WT) (DDX3X Human)
UseTags6xHis and MBPExpressionInsectMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSbi-pur-KSR1-CA3-EGFP (C-KSR)
Plasmid#217768PurposeFluorescent reporter for ceramide (for stable mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterEF-1alpha promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)
Plasmid#217767PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400PromoterT7 promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)
Plasmid#217766PurposeFluorescent reporter for ceramide (for bacterial expression and purification)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterT7 promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMS178
Plasmid#215678PurposeEmpty repair plasmid for ChrII split hygromycinR landing padDepositorInsert5'HA + MCS + loxP + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP-C1-FKBP-PIP5K1C-HD-EE
Plasmid#220090PurposeRecruitable PIP5K to make PIP2DepositorInsertPIP5K1C (PIP5K1C Human)
UseTagsTagBFP-FKBP1A(3-108)ExpressionMammalianMutationamino acids 1-635; D101R, R304D, R445E, K446EPromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_mNeonGreen_FLAG_C
Plasmid#218073PurposeRESOLUTE cloning plasmidDepositorTypeEmpty backboneUseLentiviralTagsFLAG-mNeonGreen-V5ExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TAG
Plasmid#208188Purposenonsense mistranslation, TAG stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TAG*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only
