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Showing: 1621 - 1640 of 6476 results

  2. pCsy_complex

    Plasmid
    #89232
    Purpose
    Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site
    Depositor
    Blake Wiedenheft
    Article
    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:
    Inserts
    Csy1
    Csy2
    Csy3
    Csy4
    Use
    Tags
    6xHis-TEV protease
    Expression
    Mutation
    Promoter
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89232-plasmid-map-sequence-id-177172

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  4. pCSF107mT-GATEWAY-3'-LAP tag

    Plasmid
    #67618
    Purpose
    A pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using GATEWAY cloning technology
    Depositor
    Todd Stukenberg
    Article
    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi
    Type
    Empty backbone
    Use
    In vitro transcription or translation off of an s…
    Tags
    C-termnal LAP (eGFP-TEV-S)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and SP6
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pelB-MBP-BB0323-ss

    Plasmid
    #137067
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0323: lacks the BB0323 signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137067-plasmid-map-sequence-id-291957

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  10. pET28a_6HT-TBP6.7(R49A)

    Plasmid
    #112726
    Purpose
    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.
    Depositor
    Joseph Wedekind
    Article
    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64
    Insert
    6His-TEV-TBP6.7(R49A)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    R49A
    Promoter
    T7
    Available since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112726-plasmid-map-sequence-id-216329

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  13. DYRK2_HUMAN_D0

    Plasmid
    #79729
    Purpose
    This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    Markus Seeliger, Nicholas Levinson, John Chodera
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    DYRK2 (DYRK2 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. STK16_HUMAN_D0

    Plasmid
    #79701
    Purpose
    This plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    Markus Seeliger, Nicholas Levinson, John Chodera
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    STK16 (STK16 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1621 - 1640 of 6476 results