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pETSG

Plasmid

#159418

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Use

Tags

polyhistidine (poly-His)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-tdTomato-2A-GCaMP6f

Plasmid

#80317

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6f

Use

Lentiviral

Tags

Expression

Mutation

Promoter

RSV

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-Int

Plasmid

#50000

Purpose

Expresses the integrase on a suicide plasmid

Depositor

Brian Robertson, Peter Sander

Article

Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

Insert

Integrase from pMV361

Use

Tags

Expression

Bacterial

Mutation

Promoter

own

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
PGEX-NEDD4

Plasmid

#45043

Depositor

Shiaw-Yih Lin

Article

Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

Insert

NEDD4-1 (NEDD4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211462

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET14b – lambda exonuclease

Plasmid

#104531

Purpose

bacterial expression of lambda exonuclease

Depositor

Charles Bell

Article

Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

Insert

λ exonuclease

Use

Tags

His6 followed by a thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pMP71-CD40L

Plasmid

#174595

Purpose

Stably expresses the extracellular and transmembrane domain of murine CD40L

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

CD40L

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B WT

Plasmid

#175025

Purpose

Expresses WIPI2B fused to Halo in mammalian cells

Depositor

Andrea Stavoe, Erika Holzbaur

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAD6

Plasmid

#134349

Purpose

E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

Depositor

Seth Darst

Article

Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

Insert

mfd (mfd E. coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-tdTomato-2A-GCaMP6s

Plasmid

#80316

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6s

Use

Lentiviral

Tags

Expression

Mutation

Promoter

RSV

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pNM262

Plasmid

#128412

Purpose

Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

Lysostaphin (epr Staphylococcus simulans)

Use

Synthetic Biology

Tags

amyE SP

Expression

Bacterial

Mutation

Promoter

Pspank(V)

Available since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCpfSA

Plasmid

#187890

Purpose

CRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus

Depositor

Quanjiang Ji

Article

Wang et al ACS Synth Biol. 2022 Sep 16;11(9):3049-3

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pNR291

Plasmid

#79555

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

GFPmut3b
mTagBFP
mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pUdOm1.0

Plasmid

#210298

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTR

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP P495L

Plasmid

#62232

Purpose

Mutant of hamster BiP-mGFP unable to bind clients.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Use

Tags

mGFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10

Plasmid

#211466

Purpose

Lentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

mito-mCherry-GFP1-10

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-HaloTag

Plasmid

#188907

Purpose

Expresses synaptic HaloTag

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

SYP (Syp Mouse)

Use

Lentiviral

Tags

HaloTag

Expression

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBTSG

Plasmid

#159420

Purpose

Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Use

Tags

polyhistidine (poly-His)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJK240

Plasmid

#71127

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Use

Tags

GyrA intein and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEpic

Plasmid

#84372

Purpose

3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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