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Plasmid#127032PurposeBasic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).DepositorInsertPart ?RED_NOC
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBA409
Plasmid#85971PurposeEF1a-sfGFP-Ubc-NeoDepositorInsertssfGFP
Neo
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6020nes
Plasmid#108656PurposeA JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-JNK-EV
UseTagsXFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
NID1-bio-His
Plasmid#52068PurposeExpresses full-length Nidogen-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNID1 (NID1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2_Firefly_degron-60A
Plasmid#200396PurposeA plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertFirefly luciferase - degron
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGK:TRL-EFS:iRFP670
Plasmid#68475PurposeLentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670DepositorInsertstTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEFS and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L
Plasmid#89576PurposecaCas9 integrating vector into the Neut5L locusDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
KX002: pMVP/PB-DEST
Plasmid#121869PurposepMVP destination vector, empty PiggyBac transposon vector backboneDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGoldyTALEN-VIM-TAL-2
Plasmid#62852PurposeTALEN mediated gene editing at human VIM locusDepositorInsertTALEN array
UseTALENTagsExpressionMammalianMutationPromoterminiCAGGAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGoldyTALEN-VIM-TAL-1
Plasmid#62855PurposeTALEN mediated gene editing at human VIM locusDepositorInsertTALEN array
UseTALENTagsExpressionMammalianMutationPromoterminiCAGGAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
mt deltaN20 TFB2MdeltaC3
Plasmid#205062PurposeTFB2M protein expressionDepositorInsertTFB2M (TFB2M Human)
UseTagsExpressionBacterialMutationLacks N-terminal 20 aa and C-terminal 3 aaPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-EGFP-eDHFR(69K6)
Plasmid#178850PurposeMammalian expression of EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP791_PB_dCas9-SSSavi_BirA
Plasmid#211786PurposePiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selectionDepositorInsertdCas9, Spy, Snoop, aGCN4, AviTags
UseTags3xHAExpressionMammalianMutationdCas9 D10A and H840APromoterpCAGG abd pEF1aAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet Rac1-V12
Plasmid#125136PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).DepositorInsertmNeon/mScarletP2A Rac1-V12
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarlet. 12th AA in Rac1 ch…PromoterCAGAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dMASS_1
Plasmid#209764PurposeExpression of dMASS_1 in mammalian cells under CMV promoterDepositorInsertdMASS_1
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG--uMASS_LF
Plasmid#209763PurposeExpression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoterDepositorInsertuMASS_LF
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-uMASS_1
Plasmid#209762PurposeExpression of uMASS_1 in mammalian cells under CAG promoterDepositorInsertuMASS_1
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-tdTEVDB_C-15A/G4C
Plasmid#177205PurposePermuted td intron with evolved initiation sequence for the expression of circular mRNADepositorInsertPermuted GFP with evolved initiation sequence for circular mRNA
UseTags6x HisExpressionBacterialMutationPermuted sfGFP after residue 52 & Changed Cyt…PromoteraraBADAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6085x
Plasmid#108653PurposeA tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertPicchuEV
UseLentiviralTagsXFPExpressionMammalianMutationPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
KN206: pMVP/eCFP-DEST
Plasmid#121854PurposepMVP destination vector, empty expression vector backbone w/ eCFP selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only