Addgene

We narrowed to 5,015 results for: pcdna3.1

Showing: 1621 - 1640 of 5015 results

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pcDNA3.1(-) HuTF-Fc-His tag

Plasmid

#183253

Purpose

Soluble expression of the extracellular domains of human tissue factor (TF)-Fc

Depositor

Alexander McLellan

Insert

Tissue factor (F3 Human)

Use

Tags

8xHis

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)

Plasmid

#181861

Purpose

FluoSTEP-AKAR using mScarlet-I as the FRET acceptor.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AKAR (mScarlet-I)

Use

Tags

mScarlet-I and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H

Plasmid

#177767

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

R9A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H

Plasmid

#177768

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

S95A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

Plasmid

#177769

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

K116A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

Plasmid

#177770

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

T139A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

Plasmid

#177771

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

K370A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H

Plasmid

#177772

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

S374A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

Plasmid

#177773

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

K592A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-G120E_W

Plasmid

#147819

Purpose

Mammalian Expression of HsSmg5-G120E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-G120E (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_633-1091_T

Plasmid

#147558

Purpose

Mammalian Expression of HsSmg7_633-1091

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_633-1091 (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation V854I compared to the seq…

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-TRBP-dsRBD2

Plasmid

#180602

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-TRBP-dsRBD1

Plasmid

#180601

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Arg57Ala

Plasmid

#180759

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Arg57Ala

Use

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Arg57Ala, Leu1…

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Tyr139Ala

Plasmid

#180761

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Tyr139Ala

Use

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Tyr139Ala, Leu…

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Asp115Ala

Plasmid

#180760

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Asp115Ala

Use

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Asp115Ala, Leu…

Promoter

CMV

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRBP-L2+D2+L3-myc/His

Plasmid

#180600

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBIV

Plasmid

#176055

Purpose

Expresses Myc Mutant (delta Myc BoxIV) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 304-324

Promoter

CMV

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBIIb

Plasmid

#176054

Purpose

Expresses Myc Mutant (delta Myc BoxIIb) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 259-270

Promoter

CMV

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Showing: 1621 - 1640 of 5015 results

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