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pEGFP-FCHO1

Plasmid

#196910

Purpose

visualization of clathrin-coaed vesicle formation in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

Insert

FCHO1 (FCHO1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Sec15

Plasmid

#53760

Purpose

pEGFP-C3 plasmid that expresses Sec15

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec15 (EXOC6 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pET22b-mEB2-eGFP-6xHis

Plasmid

#130982

Purpose

Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB2-GFP-6xHis (Mapre2 Mouse)

Use

Tags

eGFP-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA2m

Plasmid

#208700

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA2m

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Sec8

Plasmid

#53758

Purpose

pEGFP-C3 plasmid that expresses Sec8

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec8 (EXOC4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pBABE puro SDF-1 alpha

Plasmid

#12270

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

SDF-1 alpha (CXCL12 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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NKP66 pEGFP-N2 (1176)

Plasmid

#62039

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Insert

MARCHV (MARCHF5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-FL

Plasmid

#202515

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B N133I

Plasmid

#89449

Purpose

Expression of GFP-tagged human Rab27B N133I in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B N133I (RAB27B Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

N133I

Promoter

CMV

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PPP1R12A wt

Plasmid

#31658

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)

Use

Tags

M2

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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Basigin-isoform2-CD4d3+4-bio

Plasmid

#73129

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin isoform 2 (BSG Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

Plasmid

#177576

Purpose

Expresses SOUL in neuronal cell types

Depositor

Guoping Feng

Article

Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

Insert

SOUL

Use

AAV

Tags

tdTomato

Expression

Mutation

Promoter

hSyn

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

Plasmid

#160458

Purpose

Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Inserts

PAX7 Left/5' Homology Arm (PAX7 Human)
PAX7 Right/3' Homology Arm (PAX7 Human)

Use

Insertion of a fluorescent reporter through homol…

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Sec6

Plasmid

#53757

Purpose

pEGFP-C3 plasmid that expresses Sec6

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec6 (EXOC3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-Sec10

Plasmid

#53759

Purpose

pEGFP-C3 plasmid that expresses Sec10

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec10 (EXOC5 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2 RB1 sgRNA #1

Plasmid

#202516

Purpose

Expressing Cas9 and sgRNA targeting human RB1 gene

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

RB1 sgRNA (RB1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-INK4A-zsGreen

Plasmid

#110728

Purpose

Mammalian Expression of p16INK4A IRES zsGreen

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

p16INK4A IRES zsGreen (CDKN2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-SOUL-P2A-tdTomato-WPRE-BGHpA

Plasmid

#177577

Purpose

Expresses SOUL in Cre-positive cells

Depositor

Guoping Feng

Article

Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

Insert

SOUL

Use

AAV

Tags

tdTomato

Expression

Mutation

Promoter

EF1a

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Npl4-Strep-HA

Plasmid

#113495

Purpose

Expression of human Npl4 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Npl4 (NPLOC4 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBK086

Plasmid

#183145

Purpose

pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

Depositor

Daan Swarts

Article

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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