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pFRT_TO_FlagHA_G3BP1

Plasmid

#127106

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP1 (G3BP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4_G3BP1

Plasmid

#127104

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP1 (G3BP1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 alpha

Plasmid

#20351

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 alpha (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH00.0126

Plasmid

#64257

Purpose

Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation

Depositor

Siela Maximova, Mark Guiltinan

Article

Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E

Inserts

EGFP
neomycin phosphotransferase II

Use

Binary agrobacterium vector for plant transformat…

Tags

Expression

Plant

Mutation

Promoter

E12-omega

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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KHBD00188

Plasmid

#34340

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1034 (bcd Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 beta2

Plasmid

#20355

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 beta2 (MAPK11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 CDK9 HA (P#635)

Plasmid

#14640

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 (CDK9 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pGFP-Kif5c

Plasmid

#71853

Purpose

mammalian expression of GFP-Kif5c

Depositor

Michelle Peckham

Article

Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

Insert

Kif5c (Kif5c Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Kif5c starts at AA3

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_G3BP2_G1

Plasmid

#127112

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA G3BP2 (G3BP2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag p38 alpha

Plasmid

#20815

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pGSTag Flag p38 alpha (Mapk14 Mouse)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pCR3-FLAG-Ubc13-WT

Plasmid

#43938

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

ubiquitin-conjugating enzyme E2N (UBE2N Human)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTet-SNAP-CCCAP

Plasmid

#136798

Purpose

Mammalian expression of the centrosomal protein CCCAP N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-CCCAP (SDCCAG8 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNATM3.1/V5-His TOPO-PKCi-K274W

Plasmid

#55690

Purpose

expresses dominant negative human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

A120E and K274W

Promoter

CMV

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK3b(Glu)

Plasmid

#50449

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

CMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pCR3-FLAG-Ubc13-C87A

Plasmid

#43939

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

ubiquitin-conjugating enzyme E2N (UBE2N Human)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

C87A

Promoter

Available since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only

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3341 pcDNA3 Bcl-2 T69A S70A S87A

Plasmid

#13340

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Expression

Mammalian

Mutation

changed Threonine 69 to Alanine; changed Serine 7…

Promoter

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-Sfi1

Plasmid

#136793

Purpose

Mammalian expression of the centrosomal protein hSfi1 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-hSfi1 (SFI1 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag MKK7b1(glu)

Plasmid

#14673

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b1(Glu) (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-269Glu Thr-273Glu

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag p38 gamma (agf)

Plasmid

#20354

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 gamma(agf) (Mapk12 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

Replace Thr 183 and Tyr 185 with Ala and Phe resp…

Promoter

Available since

April 29, 2009

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-plk4

Plasmid

#136788

Purpose

Mammalian expression of the centrosomal protein plk4 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-plk4 (PLK4 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge