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Showing: 1621 - 1640 of 1937 results

  1. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

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  2. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

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  3. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Expression
    Mutation
    Promoter
    SP30
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  4. pICH-chl-roGFP2-GPXC71S

    Plasmid
    #191703
    Purpose
    Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236
    Insert
    chl-redox(ro)GFP2-GPXC71S
    Use
    Binary agrobacterium ti plasmid
    Tags
    Expression
    Plant
    Mutation
    Peroxidatic Cysteine 71 replaced with Serine
    Promoter
    CaMV 35S
    Availability
    Academic Institutions and Nonprofits only
    191703-plasmid-map-sequence-id-378859

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  5. pICH-nuc-roGFP2-GPXC71S

    Plasmid
    #191760
    Purpose
    Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236
    Insert
    nuc-roGFP2-GPXC71S
    Use
    Binary agrobacterium ti plasmid
    Tags
    Expression
    Plant
    Mutation
    GPX has no peroxidatic Cysteine 71, which is repl…
    Promoter
    CaMV 35S
    Availability
    Academic Institutions and Nonprofits only
    191760-plasmid-map-sequence-id-378856

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  11. pro::EX1-2(intΔNACs):GFP

    Plasmid
    #218570
    Purpose
    Transcriptional reporter for ARF7 promoter
    Depositor
    Anthony Bishopp
    Insert
    AT5G20730 (NPH4 Mustard Weed)
    Use
    Tags
    Expression
    Plant
    Mutation
    Mutation genomic sequence 85nt, 86nt from TA to C…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218570-plasmid-map-sequence-id-438438

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  13. pSCL475

    Plasmid
    #219503
    Purpose
    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL7
    Availability
    Academic Institutions and Nonprofits only
    219503-plasmid-map-sequence-id-435230

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Showing: 1621 - 1640 of 1937 results