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Plasmid#83950PurposeRac1 FLIM donor for dynamic imaging of GTPase activityDepositorInsertras-related C3 botulinum toxin substrate 1 (RAC1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A_BAF
Plasmid#101773PurposeExpresses BAF in human cells (with EGFP produced from the same transcript as expression control)DepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFP-P2AExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A_BAF_L58R
Plasmid#101775PurposeExpresses mutant BAF (L58R) in human cells (with EGFP produced from the same transcript as expression control)DepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFP-P2AExpressionMammalianMutationL58R mutationPromoterEF1aAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mEGFP
Plasmid#223957PurposeGateway cloning of genes of interest with an N-terminal V5-mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mEGFPExpressionMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-GRAM1b
Plasmid#211494PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP
Plasmid#85492PurposepET28a expressing codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF1A
Plasmid#172206PurposeExpresses KIF1A tagged with eGFP in mammalian cellsDepositorInsertKIF1A (Kif1a Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Sec22b
Plasmid#101918PurposeExpresses Sec22b in mammalian cellsDepositorInsertSec22b (Sec22b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
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GFP-C1-PLCdelta-PH
Plasmid#21179Purposeencodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipidsDepositorInsertPLCdelta (PLCD1 Human)
UseTagsGFPExpressionMammalianMutationPH domain of human PLC delta, amino acids Met1–Il…PromoterAvailable sinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
1GFP/RNase H1 WT
Plasmid#174447PurposeExpress GFP-tagged RNase H1 WT in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
UseTagsHis-tag, GFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt1
Plasmid#66830PurposeMammalian Expression, Bacterial ExpressionDepositorInsertE-Syt1 (ESYT1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGFP-HBx
Plasmid#24931DepositorInsertHBx
UseTagsEGFP and HAExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-GFPnanobody
Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.90 beta
Plasmid#108221PurposeMammalian expression vector for EGFP fusion to human Hsp90 betaDepositorInsertEGFP-Hsp90b (HSP90AB1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt3
Plasmid#66832PurposeMammalian Expression, Bacterial ExpressionDepositorInsertE-Syt3 (ESYT3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-PH-FAPP1
Plasmid#161986PurposeExpresses GFP-tagged PH-FAPP1. PI(4)P biosensorDepositorInsertPH-FAPP1 (PLEKHA3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
PBMBP-eGFP
Plasmid#40976DepositorInsertMBP promoter fragment (Mbp Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)3
Plasmid#127093PurposeBacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRT_htr2c(c)-GFP
Plasmid#79677Purposeserotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFPDepositorInsertserotonin receptor 2C (HTR2C Human)
UseTagsEGFP in FrameExpressionMammalianMutationsplice site of exon Vb in consensusPromoterCMVAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only