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pDD378
Plasmid#91827PurposeCodon-optimized HaloTag donor for the SapTrap cloning systemDepositorInsertHaloTag-C1
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMV762-Peredox-mCherry
Plasmid#90217PurposeFluorescent reporter for NADH:NAD+ ratios in Mycobacteria.DepositorInsertCodon Optimized Peredox-mCherry
UseShuttle vectorTagsExpressionBacterialMutationPromotergroELAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ytdTomato
Plasmid#118456PurposeOverexpression of yeast codon-optimized tdTomatoDepositorInsertytdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymScarletI
Plasmid#118452PurposeOverexpression of yeast codon-optimized mScarletIDepositorInsertymScarletI
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISV-CRISPR/Cas9PGmUbi
Plasmid#209189PurposeExpression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selectionDepositorInsertsCas9
DsRed(deltaB)
UseTagsNLSExpressionPlantMutationmissing BsaI site and plant-codon optimized with …PromoterCaMV 35S and GmUbi from Glycine max (L.) Merr. cv…Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSM-GFPnovo2
Plasmid#116943PurposeGFPnovo2_unc-54 utr vector for expression in C. elegansDepositorInsertC. elegans codon optimized GFPnovo2
UseTagsGFPnovo2ExpressionWormMutationY145F, V163A, S202T, L221VPromoternoneAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV1025
Plasmid#111424PurposeExpression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)DepositorInsertCaCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS050
Plasmid#91826PurposeCodon-optimized mScarlet-I donor for the SapTrap cloning systemDepositorInsertmScarlet-I-C1
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)
Plasmid#105922PurposeAAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITRDepositorInsertsmKate2
IRES-Multiple cloning site
UseAAVTagsExpressionMutationcodon optimizedPromoterCBh and same CBh promoter as mKate2Available sinceFeb. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
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SpCas9 iFE
Plasmid#190138PurposeExpresses SpCas9-POLB (iFE)DepositorInsertSpCas9-POLB (HiFi iFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHN872
Plasmid#178830PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsGST, His8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133639
Plasmid#152344PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJune 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRF1-GW ymVenus
Plasmid#118454PurposeOverexpression of yeast codon-optimized mVenusDepositorInsertymVenus
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3TOPO-T7-hCas9
Plasmid#161876PurposeIn vitro transcription of SpCas9DepositorInserthuman codon optimized SpCas9
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19/DdmEGFP
Plasmid#154304Purposecloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideumDepositorInsertmEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pKG113
Plasmid#63073PurposeRetroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)DepositorInsertCENP-T (CENPT Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationE. coli codon optimized. G217R mutation compared …PromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only