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Plasmid#87398PurposeAll-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1206a sequence CGAACATTTTTCCATGCGCT in yeast chromosome 12.DepositorInsertHuman Optimized S. pyogenes Cas9 and guide RNA targeting ARS1206a
UseCRISPRTagsExpressionMutationPromoterADH1, pTyrosineAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7ADelta2bp-YFP
Plasmid#62637Purposemammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationthere is a 2-bp deletion in exon 6 of CHRFAM7A an…PromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL403
Plasmid#119953PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
UseTagsExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…PromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAST-pHuji-GINKO2
Plasmid#177117PurposeGenetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusionDepositorInsertpHuji-GINKO2
UseTagsExpressionInsectMutationPromoterhsp70 promoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2XStrep-ATIC
Plasmid#125685Purposeexpresses human ATIC protein with an N-terminal 2XStrep affinity tagDepositorInsertATIC (ATIC Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT K842M (Puro)
Plasmid#154291PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationK842M mutation (Catalytic Inactive)PromoterPGKAvailable sinceDec. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Ctnnb1
Plasmid#123586DepositorInsertcatenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC7A2
Plasmid#131937PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A2 (SLC7A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL
Plasmid#119952PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
UseTagsExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…PromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-358-iRFP670
Plasmid#110694PurposeExpresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670DepositorInsertCPSF6-iRFP670 (CPSF6 Human)
UseLentiviralTagsiRFP670ExpressionMutationTruncation of CPSF6 isoform at amino acid 358PromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
CACNA1F_L (OZ507)
Plasmid#26957DepositorInsertZinc finger array targeting cacna1f (cacna1f Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
Atg7_L (OZ557)
Plasmid#28082DepositorInsertZinc finger array targeting Atg7 (atg7 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
Atg7_R (OZ558)
Plasmid#28083DepositorInsertZinc finger array targeting Atg7 (atg7 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
atp2c1_L (OZ567)
Plasmid#33381DepositorInsertZinc finger array targeting atp2c1 (atp2c1 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
CACNA1F_R (OZ508)
Plasmid#27183DepositorInsertZinc finger array targeting cacna1f (cacna1f Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
zgc:111821_R (OZ598)
Plasmid#35238DepositorInsertZinc finger array targeting zgc:111821 (zgc:111821 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
zgc:111821_L (OZ597)
Plasmid#35237DepositorInsertZinc finger array targeting zgc:111821 (zgc:111821 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
Encephalopsin (Opn3)_L (OZ541)
Plasmid#28066DepositorInsertZinc finger array targeting Encephalopsin (Opn3) (opn3 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
Encephalopsin (Opn3)_R (OZ542)
Plasmid#28067DepositorInsertZinc finger array targeting Encephalopsin (Opn3) (opn3 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lenti CGIP + M-AAT target
Plasmid#86008Purposelenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864DepositorInsertsCAG promoter
M-AAT target
UseLentiviralTagsExpressionMutationV30MPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only