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Plasmid#173009PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.DepositorInsertDHFR-mNeon-GluA1
UseTagsDHFR and mNeonExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-NLS-TIR1_P2A_NES-TIR1
Plasmid#117699PurposeExpression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNADepositorInsertsTRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1
UseTagsHA tag, Myc-NES, and NLSExpressionMammalianMutationcodon optimized for murine expressionPromoterNone, 2A peptide from porcine teschovirus-1 polyp…Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Lifeact-7
Plasmid#54663PurposeLocalization: Actin, Excitation: 604, Emission: 659DepositorTypeEmpty backboneUseTagsmCardinal-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-EB3mCherry_cmv-LifeActGFP
Plasmid#133927PurposeLive dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeletonDepositorInsertEB3-mCherry and Lifeact-GFP (MAPRE3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa-flag-BicD2 594-FKBP
Plasmid#64206PurposeBicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBPDepositorInsertBicaudal D protein (Bicd2 Mouse)
UseTagsFKBP and flagExpressionMammalianMutationno start methionine, aa1-594Promoterchicken Beta ActinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)
Plasmid#36880DepositorInsertstTA2
tdT-3Myc
insulator
UseTags3 Myc tagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF1Bbeta tail 387-1770
Plasmid#134620PurposeMammalian expression of mouse KIF1Bbeta 387-1170DepositorInsertKIF1Bbeta (Kif1b Mouse)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 386 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJR025
Plasmid#198832PurposeActin filament and cell cortex localization.DepositorInsert15xUAS::act2-venus::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF1Balpha tail 387-1150
Plasmid#134619PurposeMammalian expression of mouse KIF1Balpha 387-1150DepositorInsertKIF1Balpha (Kif1b Mouse)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 386 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIG-VP16–RAR-alpha
Plasmid#80434PurposeExpression of constitutively active human RAR-alpha in chick embryosDepositorInsertRetinoic acid receptor alpha (RARA Human)
UseTagsEGFP and VP16ExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry
Plasmid#175261Purposelabelling of microtubule plus end and F-actin structuresDepositorInsertEB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
UseTagsmCherry (on iLifeact) and mNeonGreen (on EB3)ExpressionMammalianMutationNone (wt)PromoterpTub-alpha1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH BRGI-Flag
Plasmid#19148DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS mSynCAM1
Plasmid#115496Purposeexpression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expressionDepositorInsertCadm1 (Cadm1 Mouse)
UseTagsExpressionMammalianMutationPromoterchicken beta-actinAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCIG-V5-Snail2-IRES-nls-GFP
Plasmid#44282DepositorInsertV5-Snail2 (SNAI2 Chicken)
UseTagsV5ExpressionMammalianMutationPromoterCMV-IE-enhancer and chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Venus-MDGA1
Plasmid#82543PurposeOverexpression vector for Venus-MDGA1 in neuronsDepositorInsertMDGA1 (MDGA1 Human)
UseTagsVenus fluorescent proteinExpressionMammalianMutationPromoterchicken beta actinAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG ChR2 E123T T159C 2A tDimer
Plasmid#85399PurposeHigh efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoterDepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE123T , T159CPromoterCAG and ribosomal skip sequence 2AAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Venus-MDGA2
Plasmid#82544PurposeOverexpression vector for Venus-MDGA2 in neuronsDepositorInsertMDGA2 (Mdga2 Rat)
UseTagsVenus fluorescent proteinExpressionMammalianMutationPromoterchicken beta actinAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc-y1sgRNA-Cas9
Plasmid#49331PurposeExpresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertsUseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
inhibin-DsRed
Plasmid#156184PurposeExpression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.DepositorInsertsrainbow trout inhibin α promoter
rainbow trout β actin 3'UTR
UseTagsExpressionBacterialMutationPromoterinhibinAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only