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Plasmid#219655PurposehU6-driven expression of CasRx guide RNA targeting EZH2. 5' processed DR followed by EZH2 guide.DepositorInsertCasRx gRNA targeting EZH2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTKW106alp7A
Plasmid#69360PurposeStabilized lacZ expression vector containing alp7A and hok/sokDepositorInsertslacZ
alp7A
hok
placIq-lacI
UseTagsExpressionBacterialMutationPromoterpTac and placIqAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
RepX_pET28a(+)
Plasmid#172199PurposeExpresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.DepositorInsertRepX superhelicase
UseTags6xHis tagExpressionBacterialMutationC18L, C43S, C167V, S400C, C612APromoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-FOXM1-shRNA-84
Plasmid#89494PurposeLentiviral vector with constitutive expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_MFE1
Plasmid#84612PurposeIntroduces DSB at MFE1 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shCIAP2-B
Plasmid#44132DepositorInsertCIAP2
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceMarch 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pSUPER ERbeta shRNA
Plasmid#35561DepositorInsertERbeta
UseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
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ZO-2 shRNA 2434
Plasmid#37211DepositorInsertdog ZO-2 shRNA (TJP2 canine)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
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ZO-2 shRNA 1320
Plasmid#37210DepositorInsertdog ZO-2 shRNA (TJP2 canine)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJK350
Plasmid#71560PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala365>Asp mutant (H6AaAlr-A365D)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationmutation changes alanine-365 to aspartatePromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-ARNTL-shRNA2
Plasmid#166914PurposeLentivirus for inducible knockdown of human ARNTL(BMAL1)DepositorInsertARNTL shRNA (BMAL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2
Plasmid#118159PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK290
Plasmid#71554PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with double mutant (AaAlr-K39A,Y260A)DepositorInsertalanine racemase
UseTagsExpressionBacterialMutationchanges lysine-39 to alanine and tyrosine-260 to …PromoterT7Available sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK326
Plasmid#71557PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationchanges alanine-368 to aspartatePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK325
Plasmid#71556PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationchanges alanine-367 to aspartatePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK345
Plasmid#71559PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationdeletes alanine-367 and alanine-368PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK344
Plasmid#71558PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationdeletes alanine-367 (same as deleting alanine-368)PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only