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Plasmid#60978PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic EBFP2.DepositorInsertEBFP2
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NFIA.1-SOX9
Plasmid#182310PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0532
Plasmid#133502PurposeExpression of mTagBFP2 targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
UtB2N7
Plasmid#161015Purposeubiquitously expressed nuclear localized tagBFP2DepositorInserttagBFP2
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0471
Plasmid#133490PurposeExpression of nucleo-cytoplasmic mTagBFP2DepositorInsertpUra4(AfeI)-p(tdh1*)-mTagBFP2-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0771
Plasmid#133513PurposeExpression of mTagBFP2 tagged CHD domain to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-3mTagBFP2-CHDRng2-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0624
Plasmid#133491PurposeExpression of cytoplasmic 3mTagBFP2DepositorInsertpUra4(AfeI)-p(tdh1)-3mTagBFP2-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBD21_034
Plasmid#109151PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFPDepositorInsertsmRFP
TagBFP
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0770
Plasmid#133518PurposeExpression of mTagBFP2 tagged beta-tubulin to monitor microtubulesDepositorInsertpAde6(PmeI)-p(atb2)-mTagBFP2-atb2-terminator(atb2)-hphMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0761
Plasmid#133494PurposeExpression of nucleo-cytoplasmic mTagBFP2 in P-cellsDepositorInsertpLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mB
Plasmid#60985PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EBFP2.DepositorInsertEBFP2
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2ΔEC1ΔICD-EB
Plasmid#208522PurposeEBFP2-fused PcdhγB2 lacking an EC1 domain and an ICD domainDepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsEBFP2ExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_027
Plasmid#123779PurposeEncodes tagBFP as a Type 3a part to be used in the MTK systemDepositorInserttagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_006
Plasmid#123787PurposeEncodes tagBFP as a Type 3b part to be used in the MTK systemDepositorInserttagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_008
Plasmid#123789PurposeEncodes EBFP2 as a Type 3b part to be used in the MTK systemDepositorInsertEBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0763
Plasmid#133497PurposeExpression of nucleo-cytoplasmic mTagBFP2 in M-cellsDepositorInsertpAde6(PmeI)-p(mam1*)-mTagBFP2-terminator(ScADH1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NFIA.1-SOX9 (bi-directional promoter)
Plasmid#182306PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterTRE3G-Bi-directionalAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-I-PhoCl-HCVp
Plasmid#87697PurposeExpress I-PhoCl-HCVp with N-term EBFP2 in mammalian cellsDepositorInsertI-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
UseTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK332
Plasmid#59573PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5DepositorInsertSGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK333
Plasmid#59574PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5DepositorInsertSGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only