Addgene

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psicheck-2-Mc2r

Plasmid

#48174

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of MC2R (MC2R Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
XE224 human flag-Chibby-pCS3+FLT

Plasmid

#16824

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

flag-Chibby (CBY1 Human)

Use

Xenopus expression

Tags

flag

Expression

Mutation

Promoter

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pET28a(cd96)

Plasmid

#177901

Purpose

Bacterial expression of his10-tagged human cd96 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

CD96 (aa 557-585 only) (CD96 Human)

Use

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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Opto-GPR4

Plasmid

#106001

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR4; Opto-GPR4; A3)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR4 (GPR4 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA AXL

Plasmid

#105932

Purpose

Mammalian expression of myc-His tagged AXL

Depositor

Rosa Marina Melillo

Article

Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

Insert

AXL (AXL Human)

Use

Tags

His and myc

Expression

Mammalian

Mutation

Promoter

tet-inducible CMV

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB7

Plasmid

#63108

Purpose

Expression of HSPB7 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb7 (HSPB7 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-7 aSyn C141

Plasmid

#108866

Purpose

Expresses human WT alpha synuclein with a C-terminal cysteine

Depositor

Gabriele Kaminski Schierle

Article

Stephens et al bioRxiv 2022.10.04.510646

Insert

alpha synuclein (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

C141 mutation to allow C-terminal maleimide dye l…

Promoter

T7

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-GRP78/BiP

Plasmid

#32701

Depositor

Richard C. Austin

Article

Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

Insert

GRP78/BiP (HSPA5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 15, 2013

Availability

Academic Institutions and Nonprofits only

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XRE-H2B-eGFP

Plasmid

#182294

Purpose

Fluorescent reporter for AhR activity

Depositor

Thierry Fournier, Severine Degrelle

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

Xenobiotic Response Element (CYP1A1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

XRE

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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alpha Synuclein-VC

Plasmid

#89471

Purpose

expresses human alpha Synuclein fused to the C-terminal part of Venus

Depositor

Tiago Outeiro, Bradley Hyman

Article

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Use

Tags

C-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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VN-alpha Synuclein

Plasmid

#89470

Purpose

expresses human alpha Synuclein fused to the N-terminal part of Venus

Depositor

Tiago Outeiro, Bradley Hyman

Article

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Use

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-ATF5-PROX1-HNF1A

Plasmid

#149725

Purpose

expressing three liver promoting transcription factors (ATF5, PROX1, HNF1A)

Depositor

Baisong Lu

Article

Lu et al Biomaterials. 2021 Feb;269:120668. doi:

Insert

ATF5-PROX1-HNF1A (ATF5 Human)

Use

Lentiviral

Tags

P2A, T2A

Expression

Mutation

Promoter

CMV

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKC-4.06

Plasmid

#112084

Purpose

NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)

Depositor

James Inglese

Article

Baird et al Elife. 2018 Mar 12;7. pii: 33178. doi: 1

Insert

3XFLAG firefly luciferase-beta-globin 39PTC (HBB Human, Mouse, Photinus pyralis)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pJC49

Plasmid

#133060

Purpose

Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

Depositor

Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

Tau.K18(LM)

Use

Lentiviral

Tags

mScarletI

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEB1deltaC-mCherry

Plasmid

#80603

Purpose

Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

EB1 (MAPRE1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Truncated C-terminus; EB1 coding sequence ends at…

Promoter

CMV

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE HA Chk2 WT

Plasmid

#41901

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti6-DCX-EGFP

Plasmid

#80599

Purpose

pLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

Doublecortin (DCX Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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TFORF2769

Plasmid

#143612

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

KDM5D (KDM5D Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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loxP-myctagBFP-STOP-loxP-mCherry-NTR

Plasmid

#108456

Purpose

This plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

loxP-myctagBFP-STOP-loxP-mCherry-NTR

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-p2A-CreERT2-FRT-kan-FRT

Plasmid

#108454

Purpose

This plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

mCherry-p2a-CreERT2

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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