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Plasmid#113193PurposeDR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.DepositorInsertDR-GFP
UseDonor construct with t2a-hygromycin for gene trap…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_RVFV_GP
Plasmid#207304PurposeMammalian expression of Rift valley fever virus GP proteinDepositorInsertRVFV_GP (RVFV_sM_gp1 Rift valley fever virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
B2342_BTB-mCherry
Plasmid#215095PurposeExpresses BCL6 BTB(1-129)-mCherry in mammalian cellsDepositorInsertBCL6 BTB (BCL6 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5x_UnaG
Plasmid#163125PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.DepositorInsertUnaG
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKL2351
Plasmid#199721PurposeA pVS1-based T-DNA binary vector and destination vector for Gateway cloning.DepositorInsertNPTII
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pMD2.G-VSV-G-mut
Plasmid#196508PurposeVSV-G mutant (K47Q, R354A)DepositorInsertVSV-G
UseTagsNAExpressionMammalianMutationK47Q, R354APromoterCMVAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.1-mIglc2
Plasmid#127156PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant regionDepositorInsertIglc2 (Iglc2 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
SIRT2 Flag
Plasmid#13813PurposeMammalian expression of human SIRT2 with flag tagDepositorInsertSIRT2 (SIRT2 Human)
UseTagsflagExpressionMammalianMutationTranscript variant 2PromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
Gi2-CASE
Plasmid#168121PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at C112/E115 within GNAI2PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only