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For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170
For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminator
Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.
Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.
AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
AAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.