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Plasmid#73180PurposeExpressing FKBPF36V protein in washing out shield-1 ligand for FKBPddDepositorInsertFKBP(F36V) (FKBP1A Human)
UseTagsHis 6xExpressionBacterialMutationF36VPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11b-SpyCatcher-M2e
Plasmid#165990PurposeSpyCatcher-fused influenza M2 ectodomain with removable HisTagDepositorInsertSpyCatcher-M2e
UseTagsHisTag and SpyCatcherExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28sumo-hRig-I DCARDS
Plasmid#210828Purposeprotein expression in ecoliDepositorInserthRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET246-pUC57 12xMS2V6
Plasmid#104390PurposeMS2V6 cloningDepositorInsert12xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-HIS-Tol2
Plasmid#112486Purposefunctional recombinant Tol2 protein from E.coliDepositorInsertTol2 Transposase (tpase Synthetic)
UseTagsHIS/6xExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-SerRS-His
Plasmid#124118PurposeBacterial expression plasmid for PURE system componentsDepositorInsertSeryl-tRNA ligase (serS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-His-VCre
Plasmid#123131PurposeBacterial expression plasmid for wild-type VCre with an N-terminal His-tag.DepositorInsertVCre recombinase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-GDH
Plasmid#108954Purposeheterologous expression of GDH of B. megaterium in E. coliDepositorInsertGlucose dehydrogenase IV (GDH IV)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MTF-His
Plasmid#124133PurposeBacterial expression plasmid for PURE system componentsDepositorInsertMethionyl-tRNA formyltransferase (fmt Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET16b-His-ArgRS
Plasmid#124104PurposeBacterial expression plasmid for PURE system componentsDepositorInsertArgin+I4 ligase (argS Escherichia coli)
UseTags10x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB1
Plasmid#120361PurposeProduce human recombinant TGFB1 in DE3 cellsDepositorInsertTransforming Growth Factor-β1 (TGFB1 Human, Synthetic)
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Dead
Plasmid#20859PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).DepositorInsertStreptavidin-Dead
UseTagsExpressionBacterialMutationTriple mutant of wt Streptavidin N23A,S27D,S45APromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTH
Plasmid#200158PurposeFor bacterial expression of the APOBEC1-YTH fusion protein for protein purificationDepositorInsertAPOBEC1-YTH
UseTags6xHis, HA, and Maltose Binding ProteinExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MetRS-His
Plasmid#124115PurposeBacterial expression plasmid for PURE system componentsDepositorInsertMethionine--tRNA ligase (metG Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlnRS-His
Plasmid#124108PurposeBacterial expression plasmid for PURE system componentsDepositorInsertGlutaminyl-tRNA ligase (glnS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-His-Dre
Plasmid#123130PurposeBacterial expression plasmid for wild-type Dre with an N-terminal His-tag.DepositorInsertDre recombinase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
LOV-Turbo-His6_pET21a
Plasmid#199668Purposeexpresses LOV-Turbo in bacteriaDepositorInsertLOV-Turbo
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(-30)GFP
Plasmid#62936PurposeExpression of 6xHis-(-30)GFP in bacterial cellsDepositorInsert(-30)GFP
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-AspRS-His
Plasmid#124106PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAspartate-tRNA ligase (aspS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[N150TAA]
Plasmid#164581Purposesuperfolder GFP with N150TAA mutationDepositorInsertsfGFP-150TAA
UseTags6xHisExpressionBacterialMutationN150TAA MutationPromoterT7Available sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only