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Plasmid#90482PurposeTargeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicumDepositorInsertgroup II intron and ItrA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TC-OM45-FKBPx2
Plasmid#104471PurposeIntegrating plasmid for overexpression of OM45-FKBPx2.DepositorInsertOM45p (OM45 Budding Yeast)
UseTagsFKBPx2- HAExpressionYeastMutationPromoterTPI1Available sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXY1
Plasmid#90487PurposePlasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWJ1
Plasmid#90484PurposeFor gene disruption in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorInsertpIM13 Origin
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ARF1-msGFP
Plasmid#105255PurposePlasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.DepositorInsertArf1 (ARF1 Budding Yeast)
UseTagsmsGFPExpressionYeastMutationPromoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-TLG2
Plasmid#105262PurposePlasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.DepositorInsertTlg2 (TLG2 Budding Yeast)
UseTagsiGFPExpressionYeastMutationPromoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJSM101
Plasmid#48791PurposeNHEJ reporter plasmid for 5' and 3' cohesive endsDepositorInsertsplit lacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJSM103
Plasmid#48872PurposeNHEJ reporter plasmid for blunt endsDepositorInsertsplit lacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-YPT31
Plasmid#105263PurposePlasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.DepositorInsertYpt31 (YPT31 )
UseTagsiGFPExpressionYeastMutationPromoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-KEX2-iGFPx6
Plasmid#105264PurposePlasmid for pop-in/pop-out replacement of Kex2 with Kex2-iGFP-x6.DepositorInsertKex2 portion (KEX2 )
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSADuet502
Plasmid#46373DepositorInsertNpuDnaB intein
UseTagsGB1 and H6-GB1ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRGM10
Plasmid#48790PurposeI-SceI DSB repair reporter cassetteDepositorInsertslacZ(I-SceI)
lacZ-deltaN
UseDsb repair reporterTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-5
Plasmid#221479PurposeGateway cloning of NKX2-5 overexpressionDepositorInsertNKX2-5 (NKX2-5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL1245
Plasmid#155139PurposeExpresses MlaF in E. coliDepositorInsert6xHis-TEV-mlaF
UseTagsExpressionMutationPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1246
Plasmid#155141PurposeExpresses MlaB in E. coliDepositorInsert6xHis-TEV-mlaB
UseTagsExpressionMutationPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo(Crimson)
Plasmid#140154PurposeExpresses a vacuolar cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) AcAc
Plasmid#111332PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutation3' splice site AcAcPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(AcAc)
Plasmid#111327PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutation3' splice site AcAc mutation in intronPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-iGFP-YPT52
Plasmid#105279PurposePlasmid for integration of iGFP-Ypt52 at TRP1.DepositorInsertYpt52 (YPT52 Budding Yeast)
UseTagsiGFPExpressionYeastMutationPromoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIMP1-Pthl-AbrB3647
Plasmid#80981PurposeUsed for abrB3647(CAC3647) overexpression in Clostridium acetobutylicumDepositorInsertabrB3647(CAC3647)
UseTagsExpressionBacterialMutationPromoterthl (cac2873)Available sinceSept. 7, 2016AvailabilityAcademic Institutions and Nonprofits only