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Plasmid#210593PurposeExpresses Optimal Renilla luciferase (CSC metric; Drosophila)DepositorInsertOptimal Renilla luciferase (Optimized for Drosophila, CSC metric)
UseTagsExpressionInsectMutationPromoterAc5Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_FLuc100---MS2
Plasmid#210574PurposeExpresses Optimal Firefly with 3'UTR MS2 hairpinsDepositorInsertOptimal Firefly luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_OptλHAramp-FLuc100
Plasmid#210577PurposeExpresses Optimal lambda HA-Optimal Firefly luciferaseDepositorInsertOptimal lambda HA-Optimal Firefly luciferase
UseTagslambda HAExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_NonoptλHAramp-FLuc100
Plasmid#210579PurposeExpresses Nonoptimal lambda HA-Optimal Firefly luciferaseDepositorInsertNonoptimal lambda HA-Optimal Firefly luciferase
UseLentiviral and LuciferaseTagslambda HAExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc0-pA
Plasmid#210583PurposeExpresses SM(FLAG)-nonoptimal Firefly luciferase- pADepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc100-pA
Plasmid#210584PurposeExpresses SM(FLAG)-optimal Firefly luciferase - pADepositorInsertSpaghetti monster (FLAG)-optimal FLuc
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dm_λHA-FLuc0_CSC
Plasmid#210589PurposeExpresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)DepositorInsertLambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)
UseLuciferaseTagslambda HAExpressionInsectMutationPromoterAc5Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM2
Plasmid#225289PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC311 for pJBM2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM3
Plasmid#225290PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC388 for pJBM3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5423_p3WJdB-glcO60
Plasmid#221194PurposeDNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor systemDepositorInsertT7 promoter-glcO60-3WJdB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoter with downstream glcO60 operatorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
UseTags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK621
Plasmid#196405PurposeStrong expression of firefly luciferase codon-optimized for S. rosettaDepositorInsertSro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM013_phiC31-nTRE-Ins(high)-pEF1s(orig)-H2b-Citrine
Plasmid#197681Purpose"Insert(high)-pEF1s(orig)" reporter plasmidDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM015_phiC31-nTRE-Ins(low)-pEF1s(orig)-H2b-Citrine
Plasmid#197683Purpose"Insert(low)-pEF1s(orig)" reporter plasmidDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM142_phiC31-nTRE-pEF1s(high)(CC793)-H2B-citrine
Plasmid#197685Purpose"pEF1s(high)" reporter plasmid, with CpG793 mutated to CCDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4E-I96AI112A-siRNAres_Z
Plasmid#148163PurposeInsect Expression of DmeIF4E-I96AI112ADepositorInsertDmeIF4E-I96AI112A
UseTagsExpressionInsectMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4E-W106A-siRNAres_Z
Plasmid#148164PurposeInsect Expression of DmeIF4F-W106ADepositorInsertDmeIF4F-W106A
UseTagsExpressionInsectMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-DmeIF4E_69-248_Z
Plasmid#148172PurposeBacterial Expression of DmeIF4E_69-248DepositorInsertDmeIF4E_69-248
UseTagsExpressionBacterialMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-DmeIF4E_69-248-I96AI112A_Z
Plasmid#148173PurposeBacterial Expression of DmeIF4E_69-248-I96AI112ADepositorInsertDmeIF4E_69-248-I96AI112A
UseTagsExpressionBacterialMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-FWK41AAA_T
Plasmid#147603PurposeInsect Expression of Dm4E-T-FKW41AAADepositorInsertDm4E-T-FKW41AAA (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only