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Plasmid#119211PurposeExpresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domainDepositorInsertsplit nPPVp in fusion with PYL1
UseTagsAU1 tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP3bright
Plasmid#74423PurposeExpress fluorescent calcium indicator GCaMP3bright in mammalian cellsDepositorInsertGCaMP3bright
UseTagsExpressionMammalianMutationD392APromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
rat Kv3.1-HA
Plasmid#113055PurposeExpresses rat Kv3.1 in mammalian cellsDepositorInsertrat Kv3.1 (Kcnc1 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_PPVs
Plasmid#119208PurposeCircularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with PPVp cleavage site
UseLuciferaseTagsAU1 tagExpressionMutationPromoterCMVAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP_cSuMMVp
Plasmid#119182PurposeExpresses C-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FK-506 binding protein (FKBP)DepositorInsertsplit cSuMMVp in fusion with FKBP
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSuMMVp
Plasmid#118970PurposeExpresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSuMMVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-SNAP
Plasmid#38012DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsSNAP26mExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSbMVp
Plasmid#118969PurposeExpresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSbMVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP_cSbMVp
Plasmid#118968PurposeExpresses C-terminus of soybean mosaic virus protease (SbMVp) fused to the FK-506 binding protein (FKBP)DepositorInsertsplit cSbMVp in fusion with FKBP
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-fRGECO1
Plasmid#125247PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRGECO2
UseTagsNES signalExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDx_mRuby3-SYFP2
Plasmid#189758PurposeDual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysisDepositorInsertmRuby3-SYFP2
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf-BICD2N(1-400)
Plasmid#111857PurposeN-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICD2N(1-400)
UseTagsHis-tag, SNAPf-tag, TEV site, and ZZExpressionInsectMutationOptimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-SypGFP
Plasmid#172380PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + SypEGFP
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-tdTomato-ChIEF
Plasmid#172370PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + ChIEF
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_SuMMVs
Plasmid#119210PurposeCircularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with SuMMVp cleavage site
UseLuciferaseTagsAU1 tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-fRCaMP1
Plasmid#125249PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRCaMp1
UseTagsNES signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-PA
Plasmid#175446PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-Protein A and BiP Secretion PeptideExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGREAT2
Plasmid#170890PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-EYFP
Plasmid#172383PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertEYFP
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-jGCaMP8s
Plasmid#172389PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertGCaMP8s
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only