-
Plasmid#73687PurposePunc-47::2xNLS-FLP-D5 Expression vectorDepositorInsertPunc-47::2xNLS-FLP-D5::let-858 3'-UTR
UseFlp/frtTagsExpressionWormMutationPromoterPunc-47Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW-SpyCas1
Plasmid#113248PurposeE. coli expression vector for His-MBP-S. pyogenes Cas1DepositorInsertS. pyogenes Cas1
UseTags6xHis-MBPExpressionBacterialMutationPromoterT7Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW-SpyCas2
Plasmid#113249PurposeE. coli expression vector for His-MBP-S. pyogenes Cas2DepositorInsertS. pyogenes Cas2
UseTags6xHis-MBPExpressionBacterialMutationPromoterT7Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Venus-pA_U6-BbsI (pE057)
Plasmid#149351PurposeMammalian expression vector for VenusDepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BFP-pA_U6-BbsI (pE058)
Plasmid#149352PurposeMammalian expression vector for BFPDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCP
Plasmid#204743PurposeCas9-2A-Puro expression vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-miRFP670-pA_U6-BbsI (pE059)
Plasmid#149353PurposeMammalian expression vector for miRFP670DepositorInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW092
Plasmid#113250PurposeE. coli expression vector for His-MBP-S. pyogenes Cas1 H205ADepositorInsertS. pyogenes Cas1
UseTags6xHis-MBPExpressionBacterialMutationH205APromoterT7Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW205
Plasmid#113245PurposeE. coli expression vector for IHFa D14A + IHFbDepositorInsertsUseTagsExpressionBacterialMutationD14APromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW181
Plasmid#113127PurposeE. coli expression vector for His-MBP-E. coli Cas1 R138ADepositorInsertCas1
UseTags6xHis-MBPExpressionBacterialMutationR138APromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW203
Plasmid#113243PurposeE. coli expression vector for IHFa E10A + IHFbDepositorInsertsUseTagsExpressionBacterialMutationE10APromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)
Plasmid#214885PurposeLentiviral vector encoding TMP-regulatable RfxCas13d-DD fusionDepositorInsertEF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.BSD
Plasmid#57821PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.PAC
Plasmid#58329PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP
Plasmid#86457PurposeMouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sitesDepositorInsertBFP
UseCRISPRTagsExpressionMammalianMutationPromoterPgkAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgCD71-2
Plasmid#46918PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 geneDepositorInsertsUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1
Plasmid#99690PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Mouse, Synthetic)
UseAAV and CRISPRTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2
Plasmid#99694PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)
UseAAV and CRISPRTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV PE2
Plasmid#206276PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertPE2
UseCRISPR; Recombinant baculovirus production, multiā¦TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
hU6-Sa_tracr
Plasmid#135973PurposeContains tracrRNA-hU6 sequence for double sgRNA cloningDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only