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Plasmid#104218PurposeBacterial expression of WW domain #3 from ITCHDepositorInsertITCH WW domain #3 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ITCH WW domain #4
Plasmid#104219PurposeBacterial expression of WW domain #4 from ITCHDepositorInsertITCH WW domain #4 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ITCH WW domain #1
Plasmid#104216PurposeBacterial expression of WW domain #1 from ITCHDepositorInsertITCH WW domain #1 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
DHRS11
Plasmid#39157PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDHRS11 (DHRS11 Human)
UseTagsHis TevExpressionBacterialMutationG99L100 -> AHM; codon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-gp160-754*
Plasmid#123274PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncationDepositorInsertHIV-1 (BG505) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutations; …PromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irC6kwh
Plasmid#44725DepositorInsertspCMV-D2i promoter
mCherry
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CH115gp160-QES.c14-754*
Plasmid#123282PurposeMammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopesDepositorInsertHIV-1 (CH115) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irG4kwh
Plasmid#44723DepositorInsertspCMV-D2i promoter
EGFP
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-001428-QES.c10.L544Y-754*
Plasmid#123253PurposeMammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (001428) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-ZM197M-QES.c10-754*
Plasmid#123222PurposeMammalian expression plasmid for Env from the ZM197M HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (ZM197M) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-B41gp160-V200E-754*
Plasmid#123244PurposeMammalian expression plasmid for Env from the B41 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (B41) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-25711-QES.c11-754*
Plasmid#123218PurposeMammalian expression plasmid for Env from the 25711 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (25711) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
SFG.wtCNa_opt.IRES.eGFP
Plasmid#22489DepositorInsertcodon optimised wild type Calcineurin A (PPP3CA Human)
UseRetroviralTagsExpressionMutationCodon Optimised wtCNaPromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pICSL50016
Plasmid#50315DepositorInsertCDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
UsePlant expressionTagsExpressionMutationCodon-optimised for A. thaliana. BsaI/BbsI sites …PromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc0-pA
Plasmid#210583PurposeExpresses SM(FLAG)-nonoptimal Firefly luciferase- pADepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
1457_pDEST_miniTol2_R4-R2_Cryst_mCherry
Plasmid#171796PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP
Plasmid#85492PurposepET28a expressing codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWXC078
Plasmid#222961PurposeC. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backboneDepositorInsertmScarlet-I3
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHN1002
Plasmid#178831PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, 3CExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWXC077
Plasmid#222960PurposeC. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backboneDepositorInsertmScarlet3
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only