Showing: 1601 - 1620 of 1687 results
-
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1mCNPRS
Plasmid#174718PurposetRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1pCNPRS
Plasmid#174719PurposetRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
Plasmid#174081PurposeExpression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.DepositorInsertNES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
UseTagsNES-FlagExpressionMammalianMutationL270G, Y271L, N311G, C313APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSUP-(2xCouRS-tRNAopt)
Plasmid#197930PurposeMachinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycineDepositorInsertsCouRS
tRNA opt
UseTagsExpressionBacterialMutationPromoterglnS' and lambda(ts) and proKAvailabilityAcademic Institutions and Nonprofits only -
pcPylRS(Y384F Y306A)
Plasmid#196486PurposeTo express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.DepositorInsertpyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7AW)RS
Plasmid#207516PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pIDTSmart-MbPylRS
Plasmid#217361PurposeExpresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmidDepositorInsertM. barkeri pyrrolysyl-tRNA synthetase
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAcBac1.tR4-MbPyl
Plasmid#50832Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Pyl tRNA cassette
MbPyl
two copy Pyl tRNA cassette
UseBaculoviralTagsExpressionInsectMutationPromoterCMV and U6 and H1AvailabilityAcademic Institutions and Nonprofits only -
pUltra-sY
Plasmid#82417PurposeExpresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codonsDepositorInsertMethanococcus jannaschii aaRS for sulfotyrosine
UseTagsExpressionBacterialMutationTyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…PromotertacIAvailabilityAcademic Institutions and Nonprofits only -
pDule-pCNF
Plasmid#85494PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
pDule2-pCNF
Plasmid#85495PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
pDule-IBBN (G2)
Plasmid#85500PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
pDule2-IBBN (G2)
Plasmid#85501PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
pDule-para-aminoPhe
Plasmid#85502PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
pDule2-para-aminoPhe
Plasmid#85503PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
xCas9
Plasmid#138565PurposeExpresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSDDepositorInsertxCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterEFSAvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-Mma PylRS
Plasmid#140009PurposePlasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-Mma PylRS AF
Plasmid#140023PurposePlasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationY271A, Y349FPromoterEF1AvailabilityAcademic Institutions and Nonprofits only
Showing: 1601 - 1620 of 1687 results