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pMM7

Plasmid

#201213

Purpose

Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.

Depositor

Alex Nielsen

Article

Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1

Inserts

sfGFP
Spo0A right flank
Spo0A left flank

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

F11V, T13P, N39D, A179A

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pUAST ɑSynWT-EGFP

Plasmid

#201249

Purpose

allows the expression of the insert in Drosophila melanogaster

Depositor

Pedro Domingos

Article

Pocas et al Hum Mol Genet. 2015 Apr 1;24(7):1898-907

Insert

ɑ-Synuclein (SNCA Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/MACROD2

Plasmid

#172579

Purpose

For generation of FlpIn lines with untagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS-rPALcc

Plasmid

#145804

Purpose

expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells

Depositor

Richard Mains, Betty Eipper

Article

Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9

Insert

rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)

Use

Tags

Expression

Mammalian

Mutation

changed serine 767 to alanine to prevent glycosyl…

Promoter

CMV

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

Plasmid

#120297

Purpose

AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIA4

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

Plasmid

#120300

Purpose

AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC1

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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SNTB1_PDZ_1

Plasmid

#103951

Purpose

Protein expression and purification of TAX INTERACTION PROTEIN 43 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SNTB1 (SNTB1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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USH1C_PDZ_2

Plasmid

#103937

Purpose

Protein expression and purification of P73 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

USH1C (USH1C Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM W4108A

Plasmid

#83462

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, W4107A

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

Plasmid

#83460

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, S4144D

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)

Plasmid

#83463

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4169

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS S4144D-Myc/His

Plasmid

#83459

Purpose

Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4107-4303, S4144D

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

Plasmid

#83457

Purpose

Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4127-4303

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS L4137P-GST

Plasmid

#83455

Purpose

Human PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303, L4137P

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS R4150C-GST

Plasmid

#83454

Purpose

Human PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303, R4150C

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-a4-delta tail

Plasmid

#80018

Purpose

Mammalian expression of human integrin alpha4 with no tail

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Use

Tags

Expression

Mammalian

Mutation

no cytoplasmic tail (amino acids 1 to 1007)

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3H09

Plasmid

#71003

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

SNF4Agamma (SNF4Agamma Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C07

Plasmid

#70985

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

14-3-3zeta (14-3-3zeta Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2B09

Plasmid

#70918

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

gro (gro Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C03

Plasmid

#70959

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf4 (Taf4 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only