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Showing: 4001 - 4050 of 156588 results

  5. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222497
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222497-plasmid-map-sequence-id-441134

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  6. pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid
    #215015
    Purpose
    Express an autophagy (LC3) reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    MAP1LC3B (Map1lc3b Rat)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215015-plasmid-map-sequence-id-439220

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  14. GFP-C1-PLCdelta-PH

    Plasmid
    #21179
    Purpose
    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids
    Depositor
    Tobias Meyer
    Article
    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.
    Insert
    PLCdelta (PLCD1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    PH domain of human PLC delta, amino acids Met1–Il…
    Promoter
    Available since
    July 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21179-plasmid-map-sequence-id-212104

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  16. LD-GA

    Plasmid
    #209862
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Use
    Tags
    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209862-plasmid-map-sequence-id-415467

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  18. pCI-HA-Fis1

    Plasmid
    #191947
    Purpose
    Expression of Fis1
    Depositor
    Luca Scorrano
    Insert
    Fis1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    191947-plasmid-map-sequence-id-379576

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  23. Lamp1_mStayGold

    Plasmid
    #221902
    Purpose
    Expresses mStayGold fused to Lamp1
    Depositor
    Janelia Integrative Imaging
    Insert
    Lamp1_mStayGold
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    221902-plasmid-map-sequence-id-438067

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  24. PGAL

    Plasmid
    #60337
    Purpose
    Contains full length native galactose promoter
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Inserts
    promoter
    yECitrine
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    60337-plasmid-map-sequence-id-96618

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  25. MSCV-Cre

    Plasmid
    #24064
    Depositor
    Dan Littman
    Insert
    Cre
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24064-plasmid-map-sequence-id-322907

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  28. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  35. pRosetta

    Plasmid
    #59700
    Purpose
    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.
    Depositor
    David Root, John Doench
    Inserts
    Puro
    BlastR
    EGFP
    Use
    Lentiviral
    Tags
    2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    59700-plasmid-map-sequence-id-140560

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  46. pCDB179-DepoPETase

    Plasmid
    #218702
    Purpose
    Encodes codon optimized DepoPETase enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    DepoPETase
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218702-plasmid-map-sequence-id-434087

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  48. pZP06C

    Plasmid
    #232322
    Purpose
    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.
    Depositor
    Chenggang Wu
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rpsJ
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232322-plasmid-map-sequence-id-462278

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Showing: 4001 - 4050 of 156588 results