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Plasmid#168299PurposeSecretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyADepositorInsertSegment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
UseTagsExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cloning template vector
Plasmid#131471PurposeCloning template form ORANGE method based knock-in constructsDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
phH1-gRNA
Plasmid#53186PurposeExpresses the S. pyogenes sgRNA from the H1 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman H1Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREP-8xARE-GFP-SV40-BFP
Plasmid#134910PurposeNRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFPDepositorInserts8xARE- minimal promoter - GFP
TagBFP
UseTagsExpressionMammalianMutationPromoter8xARE - minimal promoter and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY093-EFS-BCMA-Blast-WPRE
Plasmid#192194PurposeLenti-BCMA-OE-BlastDepositorInsertLenti-BCMA-OE-Blast (TNFRSF17 Synthetic)
UseLentiviral; Mammalian expressionTagsExpressionMutationNAPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
M13KO7-ΔM13-ΔgeneIII-ΔgeneVI
Plasmid#80840PurposeModified helper phageDepositorInsertsM13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFREE
Plasmid#92050PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionBacterialMutationPromoterpRhamBAD and ptetAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
htrpC6-YFP
Plasmid#21084DepositorInsertTrpC6 (TRPC6 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCT-tRNA
Plasmid#133813PurposeCRISPR-Cas9 system for genetic manipulation of Candida tropicalisDepositorInsertcassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag mTOR wt
Plasmid#26603PurposeMammalian expression of human flag-tagged mTorDepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMEH31 Lamp2b HA
Plasmid#168208PurposeMammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tagDepositorInsertLamp2b (LAMP2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-GEM-GECO1
Plasmid#32442PurposeMammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertGEM-GECO1
UseTagsExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-ExRai-AktAR2
Plasmid#184047PurposeImproved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.DepositorInsertExRai-AktAR2
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME pHluorin2
Plasmid#73794Purposeratiometric pHluorin2 sequence in a middle entry cloneDepositorInsertratiometric pHluorin2
UseMiddle entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
UseTags8 histidine tagExpressionBacterialMutationPromoterT7 promotorAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-TOMM20-N-10
Plasmid#54282PurposeLocalization: Mitochondrial Membrane, Excitation: 487, Emission: 509DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLSB-KAN
Plasmid#166700PurposeCombined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA Ser promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFP
Plasmid#32395DepositorInsertGFP
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAureus-TnCAM
Plasmid#199807Purposevector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureusDepositorInserttransposon cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_ATP1.0
Plasmid#167581PurposeExpress the ATP sensor GRAB_ATP1.0 in Cre positive cellsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSOUP
Plasmid#165419PurposeThe pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in AgrobacteriumInsertRep A
UseHelper plasmid required for replication for all p…TagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-pegRNA-puro backbone
Plasmid#214085PurposeLentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5B
Plasmid#172203PurposeExpresses KIF5B tagged with eGFP in mammalian cellsDepositorInsertKIF5B (Kif5b Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gagust
Plasmid#196054PurposeEncodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV1-hMD2
Plasmid#13028DepositorInsertMD2 (LY96 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC
Plasmid#99641PurposeBacterial expression of wildtype Rag GTPase dimerDepositorUseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCh-Climp63
Plasmid#136293Purposeexpression of Climp63DepositorInsertCytoskeletal-linking protein 63 (Ckap4 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFlagCMV2-EFP
Plasmid#12449DepositorInsertEFP (TRIM25 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)
Plasmid#160369PurposeStable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.DepositorInsertGPR143 (GPR143 Human)
UseLentiviralTagsmScarlet-3xHAExpressionMutationPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-teLuc c-myc
Plasmid#100026PurposeEnhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)DepositorInsertteLuc, a NanoLuc mutant
UseTagsc-mycExpressionMammalianMutationD19S, D85N, C164HPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro
Plasmid#188768PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Bla
Plasmid#60526PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pGP-CMV-GCaMP6s-CAAX
Plasmid#52228PurposePlasma membrane targeted GCaMP6s, calcium sensorDepositorInsertGCaMP6s-CAAX
UseTags6xHis and CAAXExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
huCaV2.2 (pSAD442-1)
Plasmid#62574PurposeHuman CaV2.2 expression in mammalian cellsDepositorInsertCACNA1B (CACNA1B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA
Plasmid#63931PurposeEmpty AAV-RAM plasmid w. multiple cloning site (MCS)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterpRAM and TREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits only